Nigericin |
| Catalog No.GC39719 |
Ionophore K+/H+, favorisant l'échange de K+/H+ à travers la membrane mitochondriale.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 28380-24-7
Sample solution is provided at 25 µL, 10mM.
Nigericin is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes[1].Nigericin can be a NLRP3 activator that induces the release of IL-1β as a NALP3-dependent manner[2]. Nigericin triggers eryptosis, an effect paralleled by ROS formation, and in part due to induction of oxidative stress. Nigericin triggers apoptosis[3].
[1]. Zotova L, et al. Novel components of an active mitochondrial K(+)/H(+) exchange. J Biol Chem. 2010 May 7;285(19):14399-414. [2]. Mariathasan S, et al.Cryopyrin activates the inflammasome in response to toxins and ATP.Nature. 2006 Mar 9;440(7081):228-32. [3]. Bissinger R, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9.
| Cas No. | 28380-24-7 | SDF | |
| Canonical SMILES | C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O)=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@]4([H])O[C@](C)([C@]5([H])O[C@]([C@@]([C@H](C[C@H]6C)C)([H])O[C@@]6(O)CO)([H])C[C@@H]5C)CC4)C1 | ||
| Formula | C40H68O11 | M.Wt | 724.96 |
| Solubility | 1.2mg/mL in DMF, 20mg/mL in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3794 mL | 6.8969 mL | 13.7939 mL |
| 5 mM | 0.2759 mL | 1.3794 mL | 2.7588 mL |
| 10 mM | 0.1379 mL | 0.6897 mL | 1.3794 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
Lysosomal accumulation and cellular pH-responsive capability of NRhD-PEG-4 NPs. (b) Fluorescence images of A549 and MCF-7 cells incubated with NRhD-PEG-4 NPs at different pH values (4.5, 5.5, 6.5, and 7.5). (c) Fluorescence quantification in (a).
For the intracellular pH-reactive test, the cells were treated with 1.0 mg/mL nigericin (GLPBIO) in a high K+ buffer of different pH values for 30 min.
Acta Biomaterialia (2022). PMID: 36087865 IF: 10.6335 -
Related Biological Data
IPI-2I cells were transfected with siR-SLC9A3, NC, pEGFP-SLC9A3 or pEGFP. (G-H) NHE3 activity were analyzed by using pH sensitive dye BCECF-AM.
Finally, the re-addition of the Na+-solution and measurement of the pH at the end of each experiment, thefluorescence ratio was calibrated to the appropriate pH using nigericin (2 μM; Glpbio,Shanghai, China)/high K+-solutions at different pH values.
Vet Microbiol 289 (2024): 109916. PMID: 38159369 IF: 3.3 -
Related Biological Data
Vitamin K3 Suppresses Pyroptosis in THP-1 Cells. Vitamin K3 (0, 5, 10, 20, and 30 μM) was used to pretreat THP-1 cells for 1 h before LPS plus nigericin (Nig) induced pyroptosis. (A) Cell viability and (B) supernatant LDH levels were quantified.
Briefly, 1 × 106/mL cells were cultured with RPMI 1640 medium containing 2% FBS overnight and then treated with 1 μg/mL LPS for 3 h, followed by 8 μg/mL Nigericin (GlpBio) treatment for 1 h.
Biol Pharm Bull 46.1 (2023): 52-60. PMID: 36288961 IF: 2.2641
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *