Doxycycline-d3 (hyclate) |
| Catalog No.GC45440 |
An internal standard for quantifying doxycycline
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Doxycycline-d3 is intended for use as an internal standard for the quantification of doxycycline by GC- or LC-MS. Doxycycline is a broad-spectrum tetracycline antibiotic.1,2 It inhibits bacterial protein synthesis by binding to ribosomes.2,3 Doxycycline also selectively inhibits human matrix metalloproteinase-8 (MMP-8) and MMP-13 over MMP-1 with 50, 60, and 5% inhibition, respectively, when used at a concentration of 30 μM.4 It can be used as a regulator for inducible gene expression systems where expression depends on either the presence (Tet-On) or absence (Tet-Off) of doxycycline.5,6 Formulations containing doxycycline have been used in the treatment of bacterial infections and the prevention of malaria.
References
1. Williamson, G.M. The in vitro activity of vibramycin (doxycycline). Chemotherapia (Basel) 13(Suppl. 1), 1-6 (1968).
2. Griffin, M.O., Ceballos, G., and Villarreal, F.J. Tetracycline compounds with non-antimicrobial organ protective properties: Possible mechanisms of action. Pharmacol. Res. 63(2), 102-107 (2011).
3. Chopra, I. Tetracycline analogs whose primary target is not the bacterial ribosome. Antimicrob. Agents Chemother. 38(4), 637-640 (1994).
4. Smith, G.N., Jr., Mickler, E.A., Hasty, K.A., et al. Specificity of inhibition of matrix metalloproteinase activity by doxycycline: Relationship to structure of the enzyme. Arthritis Rheum. 42(6), 1140-1146 (1999).
5. Gould, D.J., Berenstein, M., Dreja, H., et al. A novel doxycycline inducible autoregulatory plasmid which displays "on"/"off" regulation suited to gene therapy applications. Gene Ther. 7(24), 2061-2070 (2000).
6. Li, Z., Michael, I.P., Zhou, D., et al. Simple piggyBac transposon-based mammalian cell expression system for inducible protein production. Proc. Natl. Acad. Sci. U.S.A. 110(13), 5004-5009 (2013).
| Cas No. | SDF | ||
| Formula | 2[C22H21D3N2O8].2HCl.XH2O.C2H6O | M.Wt | 1013.9 |
| Solubility | DMSO: slightly soluble,Methanol: slightly soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9863 mL | 4.9315 mL | 9.8629 mL |
| 5 mM | 0.1973 mL | 0.9863 mL | 1.9726 mL |
| 10 mM | 0.0986 mL | 0.4931 mL | 0.9863 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)
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