>>Signaling Pathways>> Ubiquitination/ Proteasome>> Autophagy>>4E2RCat

4E2RCat

Catalog No.GC30785

4E2RCat는 eIF4E-eIF4G 상호작용의 억제제로, IC50 값은 13.5 μM입니다.

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4E2RCat Chemical Structure

Cas No.: 432499-63-3

Size 가격 재고 수량
10mM (in 1mL DMSO)
US$305.00
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1mg
US$101.00
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5mg
US$304.00
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10mg
US$459.00
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50mg
US$1,379.00
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100mg
US$1,930.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

L132 cells are treated with 12.5 μM 4E2RCat for the indicated times and are processed for annexin V/propidium iodide staining. To this end, cell medium is collected. Cells are ished with 1 mL PBS, which is collected as well, and trypsinized in 200 μL 0.05% trypsin-EDTA. Cells are pooled with previously collected supernatants and spun for 2 min at 2,000 rpm and 4°C. The cell pellet is ished with 2 mL cold PBS, followed by another spin. After the second spin, the cell pellet is resuspended in 100 μL annexin V binding buffer and propidium iodide added to a final concentration of 5 μg/mL. After the addition of 5 μL annexin V-fluorescein isothiocyanate, samples are incubated for 15 min in the dark at room temperature and diluted. Fluorescence-activated cell sorter (FACS) analyses are performed using a FACScan instrument[1].

References:

[1]. Cencic R, et al. Blocking eIF4E-eIF4G interaction as a strategy to impair coronavirus replication. J Virol. 2011 Jul;85(13):6381-9.

Background

4E2RCat는 eIF4E-eIF4G 상호작용의 억제제로, IC50 값은 13.5 μM입니다.

4E2RCat는 캡 결합 단백질인 eIF4E와 대규모 지지 단백질인 eIF4G 간 상호작용을 방지하여 캡 종속적 번역을 억제합니다. 이것은 인간 코로나 바이러스 229E(HCoV-229E) 복제를 크게 감소시키며, 감염된 세포의 비율과 세포 내외의 전파 가능한 바이러스 수치를 줄입니다. 4E2RCat는 용량 의존적으로 캡 종속적 번역을 억제하며, FF 번역은 억제하지만 EMCV IRES 기반 Ren 번역은 억제하지 않습니다. 또한 4E2RCat는 용량 및 시간에 따라 코로나 바이러스 복제를 억제합니다[1].

4E2RCat는 생체 내에서 단백질 합성을 억제하며, 이것은 증가된 세포 사망의 결과가 아닙니다[1].

References:
[1]. Cencic R, et al. Blocking eIF4E-eIF4G interaction as a strategy to impair coronavirus replication. J Virol. 2011 Jul;85(13):6381-9.

Chemical Properties

Cas No. 432499-63-3 SDF
Canonical SMILES O=C(O)C1=CC(C2=CC=C(/C=C(SC(N3CC4=CC=CC=C4)=S)/C3=O)O2)=CC=C1Cl
Formula C22H14ClNO4S2 M.Wt 455.93
Solubility DMSO : 15.5 mg/mL (34.00 mM) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.1933 mL 10.9666 mL 21.9332 mL
5 mM 0.4387 mL 2.1933 mL 4.3866 mL
10 mM 0.2193 mL 1.0967 mL 2.1933 mL
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리뷰

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Average Rating: 5 ★★★★★ (Based on Reviews and 28 reference(s) in Google Scholar.)

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