4E2RCat |
Catalog No.GC30785 |
4E2RCat는 eIF4E-eIF4G 상호작용의 억제제로, IC50 값은 13.5 μM입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 432499-63-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | L132 cells are treated with 12.5 μM 4E2RCat for the indicated times and are processed for annexin V/propidium iodide staining. To this end, cell medium is collected. Cells are ished with 1 mL PBS, which is collected as well, and trypsinized in 200 μL 0.05% trypsin-EDTA. Cells are pooled with previously collected supernatants and spun for 2 min at 2,000 rpm and 4°C. The cell pellet is ished with 2 mL cold PBS, followed by another spin. After the second spin, the cell pellet is resuspended in 100 μL annexin V binding buffer and propidium iodide added to a final concentration of 5 μg/mL. After the addition of 5 μL annexin V-fluorescein isothiocyanate, samples are incubated for 15 min in the dark at room temperature and diluted. Fluorescence-activated cell sorter (FACS) analyses are performed using a FACScan instrument[1]. |
References: [1]. Cencic R, et al. Blocking eIF4E-eIF4G interaction as a strategy to impair coronavirus replication. J Virol. 2011 Jul;85(13):6381-9. |
4E2RCat는 eIF4E-eIF4G 상호작용의 억제제로, IC50 값은 13.5 μM입니다.
4E2RCat는 캡 결합 단백질인 eIF4E와 대규모 지지 단백질인 eIF4G 간 상호작용을 방지하여 캡 종속적 번역을 억제합니다. 이것은 인간 코로나 바이러스 229E(HCoV-229E) 복제를 크게 감소시키며, 감염된 세포의 비율과 세포 내외의 전파 가능한 바이러스 수치를 줄입니다. 4E2RCat는 용량 의존적으로 캡 종속적 번역을 억제하며, FF 번역은 억제하지만 EMCV IRES 기반 Ren 번역은 억제하지 않습니다. 또한 4E2RCat는 용량 및 시간에 따라 코로나 바이러스 복제를 억제합니다[1].
4E2RCat는 생체 내에서 단백질 합성을 억제하며, 이것은 증가된 세포 사망의 결과가 아닙니다[1].
References:
[1]. Cencic R, et al. Blocking eIF4E-eIF4G interaction as a strategy to impair coronavirus replication. J Virol. 2011 Jul;85(13):6381-9.
Cas No. | 432499-63-3 | SDF | |
Canonical SMILES | O=C(O)C1=CC(C2=CC=C(/C=C(SC(N3CC4=CC=CC=C4)=S)/C3=O)O2)=CC=C1Cl | ||
Formula | C22H14ClNO4S2 | M.Wt | 455.93 |
Solubility | DMSO : 15.5 mg/mL (34.00 mM) | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.1933 mL | 10.9666 mL | 21.9332 mL |
5 mM | 0.4387 mL | 2.1933 mL | 4.3866 mL |
10 mM | 0.2193 mL | 1.0967 mL | 2.1933 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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