GLPBio

POM 1

Catalog No.: GC17027Purity:>98.00%Synonyms: Sodium Metatungstate, Sodium Polyoxotungstate
COASDSData Sheet
POM 1 is a nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitor with Ki values of 2.58μM, 3.26μM and 28.8μM for NTPDase1 (CD39), NTPDase3 and NTPDase2, respectively.

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POM 1
Cas No.: 12141-67-2
50mgIn Stock
$72.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

1 publicationsPublications from researchers using this product

Description

POM 1 is a nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitor with Ki values of 2.58μM, 3.26μM and 28.8μM for NTPDase1 (CD39), NTPDase3 and NTPDase2, respectively[1]. POM 1 can inhibit ATP decomposition, but also blocks central synaptic transmission, an effect that is independent of NTPDase inhibition[2]. POM 1 is an inorganic compound that can be used to make heavy liquids and is widely used for density separation[3].

In vitro, POM 1 (100μM) treatment of macrophages for 24h inhibited intracellular ectonucleotidase activity, inhibited the increase in intracellular free Ca2+ caused by nucleotides, inhibited ATP-induced P2X7-related cell pyroptosis, and inhibited LPS-induced NO production[4]. POM 1 (5μM) treatment of H1299 and A549 cells for 24h significantly increased lipid peroxide levels and increased the expression of ferroptosis-related proteins[5].

In vivo, intraperitoneal injection of POM 1 (5mg/kg) in multiple myeloma (MM) model mice significantly reduced spleen weight and the number of tumor cells in the spleen[6]. Intraperitoneal injection of POM 1 (250μg) in CD39-deficient and wild-type mice significantly increased the number of natural killer (NK) cells and enhanced effector function[7].

References:
[1] Müller C E, Iqbal J, Baqi Y, et al. Polyoxometalates—a new class of potent ecto-nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitors[J]. Bioorganic & medicinal chemistry letters, 2006, 16(23): 5943-5947.
[2] Wall M J, Wigmore G, Lopatář J, et al. The novel NTPDase inhibitor sodium polyoxotungstate (POM-1) inhibits ATP breakdown but also blocks central synaptic transmission, an action independent of NTPDase inhibition[J]. Neuropharmacology, 2008, 55(7): 1251-1258.
[3] Savage N M. The use of sodium polytungstate for conodont separations[J]. Journal of Micropalaeontology, 1988, 7(1): 39-40.
[4] Pimenta-dos-Reis G, Torres E J L, Quintana P G, et al. POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages[J]. Purinergic Signalling, 2017, 13: 611-627.
[5] Lin J W, Zhou Y, Xiao H P, et al. Antitumor effects of a Sb-rich polyoxometalate on non-small-cell lung cancer by inducing ferroptosis and apoptosis[J]. Chemical Science, 2024, 15(37): 15367-15376.
[6] Yang R, Elsaadi S, Misund K, et al. Conversion of ATP to adenosine by CD39 and CD73 in multiple myeloma can be successfully targeted together with adenosine receptor A2A blockade[J]. Journal for immunotherapy of cancer, 2020, 8(1).
[7] Zhang H, Vijayan D, Li X Y, et al. The role of NK cells and CD39 in the immunological control of tumor metastases[J]. Oncoimmunology, 2019, 8(6): e1593809.

Protocol

Cell experiment [1]:

Cell lines

Macrophages

Preparation Method

Macrophages (5×105/well plated in 96-well plates) were treated or not with LPS (at the concentrations indicated) for 24h, with or without of 100μM POM 1. After this time, the culture supernatants were collected for measurement of the amount of nitrite using the Griess colorimetric method.

Reaction Conditions

100μM; 24h

Applications

POM-1 inhibits LPS-induced NO production.
Animal experiment [2]:

Animal models

C57BL/KalwRij mice

Preparation Method

Mice were injected intravenously on day 0 with 2×105 5T33 MM cells and treated intraperitoneally with 10mg/kg monoclonal anti-CD73 antibody on days 2, 4, 8, 11, 15, and 18. Of note, 5mg/kg of POM 1 was injected intraperitoneally once per day from 0 to day 4, day 7-11, and day 14-18. Number of tumor cells in the spleen was determined using FACS analysis.

Dosage form

5mg/kg; i.p.

Applications

Mice treated with the CD39 inhibitor POM 1 had significantly reduced spleen weight and fewer tumor cells in the spleen.

References:
[1]Pimenta-dos-Reis G, Torres E J L, Quintana P G, et al. POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages[J]. Purinergic Signalling, 2017, 13: 611-627.
[2]Yang R, Elsaadi S, Misund K, et al. Conversion of ATP to adenosine by CD39 and CD73 in multiple myeloma can be successfully targeted together with adenosine receptor A2A blockade[J]. Journal for immunotherapy of cancer, 2020, 8(1).

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Biological Data

1 / 1

Chemical Properties

CAS No.
12141-67-2
Synonyms
Sodium Metatungstate, Sodium Polyoxotungstate
SMILES
[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].O.[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O-].[O-].[O-].[O-].[O-].[O-].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W]
Molecular Formula
H2O40Na6W12
Molecular Weight
2986.01
Solubility
≥ 149.3mg/mL in DMSO with ultrasonic
≥50mg/mL in Water
Storage
Store at RT
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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