POM 1 (Synonyms: Sodium Metatungstate, Sodium Polyoxotungstate) |
Catalog No.GC17027
One-Click Copy Product Info
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POM 1 is a nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitor with Ki values of 2.58μM, 3.26μM and 28.8μM for NTPDase1 (CD39), NTPDase3 and NTPDase2, respectively.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 12141-67-2
Sample solution is provided at 25 µL, 10mM.
POM 1 is a nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitor with Ki values of 2.58μM, 3.26μM and 28.8μM for NTPDase1 (CD39), NTPDase3 and NTPDase2, respectively[1]. POM 1 can inhibit ATP decomposition, but also blocks central synaptic transmission, an effect that is independent of NTPDase inhibition[2]. POM 1 is an inorganic compound that can be used to make heavy liquids and is widely used for density separation[3].
In vitro, POM 1 (100μM) treatment of macrophages for 24h inhibited intracellular ectonucleotidase activity, inhibited the increase in intracellular free Ca2+ caused by nucleotides, inhibited ATP-induced P2X7-related cell pyroptosis, and inhibited LPS-induced NO production[4]. POM 1 (5μM) treatment of H1299 and A549 cells for 24h significantly increased lipid peroxide levels and increased the expression of ferroptosis-related proteins[5].
In vivo, intraperitoneal injection of POM 1 (5mg/kg) in multiple myeloma (MM) model mice significantly reduced spleen weight and the number of tumor cells in the spleen[6]. Intraperitoneal injection of POM 1 (250μg) in CD39-deficient and wild-type mice significantly increased the number of natural killer (NK) cells and enhanced effector function[7].
References:
[1] Müller C E, Iqbal J, Baqi Y, et al. Polyoxometalates—a new class of potent ecto-nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitors[J]. Bioorganic & medicinal chemistry letters, 2006, 16(23): 5943-5947.
[2] Wall M J, Wigmore G, Lopatář J, et al. The novel NTPDase inhibitor sodium polyoxotungstate (POM-1) inhibits ATP breakdown but also blocks central synaptic transmission, an action independent of NTPDase inhibition[J]. Neuropharmacology, 2008, 55(7): 1251-1258.
[3] Savage N M. The use of sodium polytungstate for conodont separations[J]. Journal of Micropalaeontology, 1988, 7(1): 39-40.
[4] Pimenta-dos-Reis G, Torres E J L, Quintana P G, et al. POM-1 inhibits P2 receptors and exhibits anti-inflammatory effects in macrophages[J]. Purinergic Signalling, 2017, 13: 611-627.
[5] Lin J W, Zhou Y, Xiao H P, et al. Antitumor effects of a Sb-rich polyoxometalate on non-small-cell lung cancer by inducing ferroptosis and apoptosis[J]. Chemical Science, 2024, 15(37): 15367-15376.
[6] Yang R, Elsaadi S, Misund K, et al. Conversion of ATP to adenosine by CD39 and CD73 in multiple myeloma can be successfully targeted together with adenosine receptor A2A blockade[J]. Journal for immunotherapy of cancer, 2020, 8(1).
[7] Zhang H, Vijayan D, Li X Y, et al. The role of NK cells and CD39 in the immunological control of tumor metastases[J]. Oncoimmunology, 2019, 8(6): e1593809.
| Cell experiment [1]: | |
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Cell lines |
Macrophages |
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Preparation Method |
Macrophages (5×105/well plated in 96-well plates) were treated or not with LPS (at the concentrations indicated) for 24h, with or without of 100μM POM 1. After this time, the culture supernatants were collected for measurement of the amount of nitrite using the Griess colorimetric method. |
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Reaction Conditions |
100μM; 24h |
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Applications |
POM-1 inhibits LPS-induced NO production. |
| Animal experiment [2]: | |
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Animal models |
C57BL/KalwRij mice |
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Preparation Method |
Mice were injected intravenously on day 0 with 2×105 5T33 MM cells and treated intraperitoneally with 10mg/kg monoclonal anti-CD73 antibody on days 2, 4, 8, 11, 15, and 18. Of note, 5mg/kg of POM 1 was injected intraperitoneally once per day from 0 to day 4, day 7-11, and day 14-18. Number of tumor cells in the spleen was determined using FACS analysis. |
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Dosage form |
5mg/kg; i.p. |
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Applications |
Mice treated with the CD39 inhibitor POM 1 had significantly reduced spleen weight and fewer tumor cells in the spleen. |
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References: |
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| Cas No. | 12141-67-2 | SDF | |
| Synonyms | Sodium Metatungstate, Sodium Polyoxotungstate | ||
| Canonical SMILES | [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].O.[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O].[O-].[O-].[O-].[O-].[O-].[O-].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W].[W] | ||
| Formula | H2O40Na6W12 | M.Wt | 2986.01 |
| Solubility |
≥ 149.3mg/mL in DMSO with ultrasonic ≥50mg/mL in Water |
Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 334.9 μL | 1.6745 mL | 3.349 mL |
| 5 mM | 67 μL | 334.9 μL | 669.8 μL |
| 10 mM | 33.5 μL | 167.4 μL | 334.9 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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Purity: >98.00%
- COA (Certificate Of Analysis)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)