OUL312 is a potent PARP10 inhibitor ( IC50 = 20 nM), showing more than 75-fold greater selectivity for PARP15 ( IC50 = 1500 nM). OUL312 is also at least 18-fold higher than all other human enzymes in the family. OUL312 has a good ADME profile, is able to enter cells, is not toxic, and effectively rescues human cells from PARP10 -induced apoptosis at sub-micromolar concentrations. OUL312 can be used for the study of cervical cancer [1].
In Vitro, OUL312 (Compound 15) (0.05-10 μM, 10-12 days) shows no significant cytotoxicity in HeLa Flp-In TRex cells and could rescue Doxycycline -induced HeLa Flp-In TRex cells in a concentration-dependent manner[1].
References:
[1]. Alaviuhkola J, et al. Optimization of 2,3-dihydrophthalazine-1,4-dione PARP inhibitor scaffold for nanomolar potency and specificity towards human PARP10. Eur J Med Chem. 2025 Dec 15;300:118111.
In Vitro, OUL312 (Compound 15) (0.05-10 μM, 10-12 days) shows no significant cytotoxicity in HeLa Flp-In TRex cells and could rescue Doxycycline -induced HeLa Flp-In TRex cells in a concentration-dependent manner[1].
References:
[1]. Alaviuhkola J, et al. Optimization of 2,3-dihydrophthalazine-1,4-dione PARP inhibitor scaffold for nanomolar potency and specificity towards human PARP10. Eur J Med Chem. 2025 Dec 15;300:118111.