Puerarin
Catalog No.: GN10680Purity:>98.00%Synonyms: Kakonein, NPI 031G
Puerarin is a phytoestrogen extracted from Pueraria lobata with protective functions on the cardiovascular system.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3681-99-0
10mM (in 1mL DMSO)In Stock
$37.00
1
5mgIn Stock
$25.00
1
10mgIn Stock
$34.00
1
25mgIn Stock
$47.00
1
50mgIn Stock
$62.00
1
100mgIn Stock
$80.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $37.00 | In Stock | 1 | |
| 5mg | $25.00 | In Stock | 1 | |
| 10mg | $34.00 | In Stock | 1 | |
| 25mg | $47.00 | In Stock | 1 | |
| 50mg | $62.00 | In Stock | 1 | |
| 100mg | $80.00 | In Stock | 1 |
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Citations
1 publicationsPublications from researchers using this productDescription
Puerarin is a phytoestrogen extracted from Pueraria lobata with protective functions on the cardiovascular system[1]. Puerarin exerts vasodilator effects on rat thoracic aorta by activating large conductance voltage-activated and calcium-activated potassium channels (BKCa)[2]. Puerarin has been widely used to delay the progression of hepatitis, liver fibrosis, and non-alcoholic fatty liver disease in animal models of liver disease[3].
In vitro, Puerarin treatment for 24 hours significantly inhibited the activity of SH-SY5Y cells with an IC50 value of 174.4μM[4]. Treatment of U251 and U87 cells with 200μM Puerarin for 48 hours significantly inhibited cell proliferation, induced cell apoptosis, and promoted the expression of Bax and cleaved caspase-3[5]. Puerarin treatment (20μM) for 48 hours promoted the proliferation of MC3T3-E1 cells, increased alkaline phosphatase (ALP) activity, and up-regulated the expression of osteogenesis-related proteins[6].
In vivo, Puerarin treatment (80mg/kg/day; i.p.) for three months improved myocardial structure and function, alleviated cardiac morphological disorder and collagen fiber proliferation in spontaneously hypertensive rats[7]. Oral administration of Puerarin (100mg/kg/day) for 28 days ameliorated Aβ(1-42) -induced cognitive impairment and reversed hippocampal cell apoptosis in rats[8]. Daily intraperitoneal injection of Puerarin (100mg/kg/day) for 13 weeks prevented high-fat diet (HFD)-induced obesity and steatosis in mice, significantly altered the composition of gut microbiota, and greatly increased the abundance of Akkermansia muciniphil [9].
References:
[1] Shu-Yong W E I, Yi C, Xiao-Yu X U. Progress on the pharmacological research of puerarin: a review[J]. Chinese Journal of Natural Medicines, 2014, 12(6): 407-414.
[2] Zhou Y X, Zhang H, Peng C. Puerarin: a review of pharmacological effects[J]. Phytotherapy Research, 2014, 28(7): 961-975.
[3] Wang D, Bu T, Li Y, et al. Pharmacological activity, pharmacokinetics, and clinical research progress of puerarin[J]. Antioxidants, 2022, 11(11): 2121.
[4] Sui X, Liu T, Zou Z, et al. Effects and mechanisms of puerarin against neuroblastoma: insights from bioinformatics and in vitro experiments[J]. BMC Complementary Medicine and Therapies, 2024, 24(1): 257.
[5] Yang J A, Li J Q, Shao L M, et al. Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines[J]. International journal of clinical and experimental medicine, 2015, 8(6): 10132.
[6] Wang N, Wang X, Cheng W, et al. Puerarin promotes osteogenesis and inhibits adipogenesis in vitro[J]. Chinese Medicine, 2013, 8(1): 17.
[7] Yan J, Honglei Y, Yun W, et al. Puerarin ameliorates myocardial remodeling of spontaneously hypertensive rats through inhibiting TRPC6-CaN-NFATc3 pathway[J]. European Journal of Pharmacology, 2022, 933: 175254.
[8] Li J, Wang G, Liu J, et al. Puerarin attenuates amyloid-beta-induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo[J]. European Journal of Pharmacology, 2010, 649(1-3): 195-201.
[9] Wang L, Wu Y, Zhuang L, et al. Puerarin prevents high-fat diet-induced obesity by enriching Akkermansia muciniphila in the gut microbiota of mice[J]. PLoS One, 2019, 14(6): e0218490.
Protocol
| Cell experiment [1]: | |
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Cell lines |
SH-SY5Y cells |
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Preparation Method |
SH-SY5Y cells were cultured in DMEM medium containing 10% fetal bovine serum at 37°C and 5% CO2, and passaged when the cell confluence reached 90%. SH-SY5Y cells were seeded in 96-well plates at a density of 5×103 cells/well and cultured in complete medium for 24h. Subsequently, Puerarin was added to the medium so that the final concentration of Puerarin was 0, 25, 50, 75, 100, 150, and 200μM, and the culture was continued for 24h. 10μl CCK-8 solution was added to each well and incubated for 2h at 37°C. The absorbance at 450nm was measured using a microplate reader. |
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Reaction Conditions |
0, 25, 50, 75, 100, 150, and 200μM; 24h |
|
Applications |
Puerarin significantly inhibited the viability of SH-SY5Y cells in a dose-dependent manner. |
| Animal experiment [2]: | |
|
Animal models |
Male spontaneously hypertensive rats |
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Preparation Method |
Thirty male specific pathogen-free (SPF) spontaneously hypertensive rats (SHR) were housed in SPF-grade animal centers under a 12h-12h light-dark cycle with ad libitum access to food and water. After acclimatization for one week, 30 rats were randomly divided into 2 groups: SHR group (saline treatment); SHR+Puerarin group (Puerarin treatment at 80mg/kg/day; i.p.). The experimental period was 3 months, after which the cardiac function of the rats was analyzed. |
|
Dosage form |
80mg/kg/day for 3 months; i.p. |
|
Applications |
Puerarin treatment improved myocardial structure and function, ameliorated cardiac morphological disorder and collagen fiber proliferation in rats. |
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References: |
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Product Documents
Purity:>98.00%Appearance:A solid
Chemical Properties
CAS No.
3681-99-0
Synonyms
Kakonein, NPI 031G
Chemical Name
7-hydroxy-3-(4-hydroxyphenyl)-8-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]chromen-4-one
SMILES
C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3C4C(C(C(C(O4)CO)O)O)O)O)O
Molecular Formula
C21H20O9
Molecular Weight
416.38
Solubility
DMF: 16 mg/ml,DMSO: 12.5 mg/ml,Ethanol: 5 mg/ml,PBS (pH 7.2): 0.25 mg/ml
Storage
Store at 2-8°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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