Name: Sulforaphane
Brand: GlpBio
SKU: GC14016
Availability: InStock
Rating: 5 (23 reviews)

Product has been cited by 2 publications

Product Documents

Quality Control & SDS

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Purity: >97.00%

Protocol

Cell experiment [1]:
Cell lines	HepaRG cells
Preparation Method	HepaRG cells at a concentration of 1.5 104 cells/mL were seeded on uncoated plastic tissue culture dishes and treated with sulforaphane at different concentrations (0 -40 uM) over a time course (12-48 h). The cells as well as the homogenates from liver tissues were solubilized, and the activity solution including a reporter dye were added to the supernatants.
Reaction Conditions	0 -40 uM sulforaphane for 12-48 h
Applications	Sulforaphane amplifies ALDH2 activity in HepaRG cells and suppresses acetaldehyde-induced proliferation and activation in LX-2 cells.
Animal experiment [2]:
Animal models	Ten-week-old female C57BL/6J mice
Preparation Method	Mice in the CD/ CCl4 group were fed the normal liquid diet and received 1 mL/kg body weight CCl4 dissolved in corn oil by intraperitoneal injection twice weekly. Mice in the ED/ CCl4 group were fed a 2.5% (v/v) ethanol-containing Lieber-DeCarli liquid diet (Research Diets) (ED) and received intraperitoneal CCl4 injection twice weekly (1 mL/kg body weight). Mice in the ED/ CCl4/SFN group were fed the ED with sulforaphane and received intraperitoneal CCl4 injection (1 mL/kg body weight) twice weekly; in this group, sulforaphane was administered peroral as a mixture of ED (5 µmoL/d/body weight).
Dosage form	Sulforaphane was administered peroral as a mixture of ED (5 µmoL/d/body weight) twice weekly
Applications	Sulforaphane exerts antioxidative effects with ALDH2 induction in liver fibrosis induced by ethanol exposure in CCl4-treated mice.
References: [1]. Ishida K, Kaji K, et,al. Sulforaphane ameliorates ethanol plus carbon tetrachloride-induced liver fibrosis in mice through the Nrf2-mediated antioxidant response and acetaldehyde metabolization with inhibition of the LPS/TLR4 signaling pathway. J Nutr Biochem. 2021 Mar;89:108573. doi: 10.1016/j.jnutbio.2020.108573. Epub 2020 Dec 31. PMID: 33388347.

Sulforaphane (SFN) known as [1-isothiocyanato-4-(methylsulfinyl)butane]. It is an isothiocyanate that is present naturally in widely consumed vegetables and has a particularly high concentration in broccoli^[1,10]. Sulforaphane is a phytocompound with antioxidant, anti-inflammatory, and antiapoptotic effects^[2].

Sulforaphane amplifies ALDH2 activity in HepaRG cells and suppresses acetaldehyde-induced proliferation and activation in LX-2 cells^[3]. Sulforaphane reversed mesenchymal-like changes induced by TGF-β1 and restored cells to their epithelial-like morphology. The expression of the epithelial marker, E-cadherin, increased after Sulforaphane treatment, while expression of the mesenchymal markers, N-cadherin, vimentin, and α-SMA decreased in A549 cells after SFN treatment^[4]. Sulforaphane inhibited cell growth and reduced collagen at the mRNA and protein levels in keloid fibroblasts. Moreover, sulforaphane markedly suppressed the expression of IL-6 and α-SMA and inhibited Stat3 and Smad3 signaling pathways in keloid fibroblast KF112 cells^[8]. Sulforaphane inhibited XWLC-05 cell growth with inhibitory concentration (IC)50 of 4.04, 3.38, and 3.02 µg/mL at 24, 48, and 72 hours, respectively. Sulforaphane affected the XWLC-05 cell cycle as cells accumulated in the G2/M phase^[9].

Sulforaphane exerts antioxidative effects with ALDH2 induction in liver fibrosis induced by ethanol exposure in CCl4-treated mice^[3]. Nrf2 plays the indispensable role for Sulforaphane cardiac protection from T2DM with significant induction of MT and other antioxidants. MT expression induced by Sulforaphane is Nrf2 dependent, but is not indispensable for SFN-induced cardiac protection from T2DM^[5]. Sulforaphane significantly reduced the incidence and size of 4NQO-induced tongue tumors in mice^[6]. Sulforaphane suppresses skin cancer via blocking sulfatase-2 with subsequent elevation in HSPGs and reduction in glypican-3. Moreover, sulforaphane attenuated skin cancer-induced activation of inflammatory and apoptotic pathways^[7].

References:
[1]. Panjwani AA, Liu H, et,al. Crucifers and related vegetables and supplements for neurologic disorders: what is the evidence? Curr Opin Clin Nutr Metab Care. 2018 Nov;21(6):451-457. doi: 10.1097/MCO.0000000000000511. PMID: 30199394.
[2]. Schepici G, Bramanti P, et,al. Efficacy of Sulforaphane in Neurodegenerative Diseases. Int J Mol Sci. 2020 Nov 16;21(22):8637. doi: 10.3390/ijms21228637. PMID: 33207780; PMCID: PMC7698208.
[3]. Ishida K, Kaji K, et,al. Sulforaphane ameliorates ethanol plus carbon tetrachloride-induced liver fibrosis in mice through the Nrf2-mediated antioxidant response and acetaldehyde metabolization with inhibition of the LPS/TLR4 signaling pathway. J Nutr Biochem. 2021 Mar;89:108573. doi: 10.1016/j.jnutbio.2020.108573. Epub 2020 Dec 31. PMID: 33388347.
[4]. Kyung SY, Kim DY, et,al. Sulforaphane attenuates pulmonary fibrosis by inhibiting the epithelial-mesenchymal transition. BMC Pharmacol Toxicol. 2018 Apr 2;19(1):13. doi: 10.1186/s40360-018-0204-7. PMID: 29609658; PMCID: PMC5879815.
[5]. Gu J, Cheng Y, et,al. Metallothionein Is Downstream of Nrf2 and Partially Mediates Sulforaphane Prevention of Diabetic Cardiomyopathy. Diabetes. 2017 Feb;66(2):529-542. doi: 10.2337/db15-1274. Epub 2016 Nov 30. PMID: 27903744; PMCID: PMC5248986.
[6]. Bauman JE, Zang Y, et,al. Prevention of Carcinogen-Induced Oral Cancer by Sulforaphane. Cancer Prev Res (Phila). 2016 Jul;9(7):547-57. doi: 10.1158/1940-6207.CAPR-15-0290. Epub 2016 Jun 23. PMID: 27339168; PMCID: PMC4930727.
[7]. Alyoussef A, Taha M. Antitumor activity of sulforaphane in mice model of skin cancer via blocking sulfatase-2. Exp Dermatol. 2019 Jan;28(1):28-34. doi: 10.1111/exd.13802. Epub 2018 Dec 11. PMID: 30315662.
[8]. Kawarazaki A, Horinaka M, et,al. Sulforaphane suppresses cell growth and collagen expression of keloid fibroblasts. Wound Repair Regen. 2017 Apr;25(2):224-233. doi: 10.1111/wrr.12512. Epub 2017 Feb 20. PMID: 28120534.
[9]. Zhou L, Yao Q, et,al. Sulforaphane-induced apoptosis in Xuanwei lung adenocarcinoma cell line XWLC-05. Thorac Cancer. 2017 Jan;8(1):16-25. doi: 10.1111/1759-7714.12396. Epub 2016 Nov 23. PMID: 27878984; PMCID: PMC5217876.
[10].Gamet-Payrastre L, Li P, et,al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33. PMID: 10728709.

Cas No.	4478-93-7	SDF
Synonyms	SFN
Chemical Name	1-isothiocyanato-4-(methylsulfinyl)-butane
Canonical SMILES	CS(CCCCN=C=S)=O
Formula	C₆H₁₁NOS₂	M.Wt	177.3
Solubility	≥ 67.6mg/mL in DMSO	Storage	Store at -20°C, sealed storage, away from moisture and light
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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		1 mg	5 mg	10 mg
	1 mM	5.6402 mL	28.2008 mL	56.4016 mL
	5 mM	1.128 mL	5.6402 mL	11.2803 mL
	10 mM	0.564 mL	2.8201 mL	5.6402 mL

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