BO3482 |
رقم الكتالوجGC32349
One-Click Copy Product Info
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BO3482 له نشاط مضاد للميكروبات ويمكن أن يمنع نمو المكورات العنقودية المقاومة للميثيسيلين (MRS) مع MIC90 من 6.25 مجم / مل
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 198013-53-5
Sample solution is provided at 25 µL, 10mM.
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BO3482 has Antimicrobial activity and can inhibit the growth of methicillin-resistant Staphylococci (MRS) with an MIC90 of 6.25 mg/mL.
In the thigh infection model with a homogeneous MRSA strain, the bacterial counts in tissues treated with BO3482-cilastatin are significantly reduced in a dose-dependent manner compare with the counts in those treat with vancomycin and imipenem-cilastatin[1].
[1]. Adachi Y, et al. In vitro evaluation of BO-3482, a novel dithiocarbamate carbapenem with activity against methicillin-resistant staphylococci. Antimicrob Agents Chemother. 1997 Oct;41(10):2282-5. [2]. Nagano R, et al. Therapeutic efficacy of BO-3482, a novel dithiocarbamate carbapenem, in mice infected with methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1997 Oct;41(10):2278-81.
Animal experiment: | In the thigh infection model, four immunosuppressed mice are used per group. An overnight culture of MRSA BB6294 in tryptic soy broth is washed and resuspended in fresh tryptic soy broth. Of this suspension, 0.1 mL is injected into the right thighs of slightly anesthetized mice. The mice receive two doses of drug subcutaneously 2 and 6 h after injection of the test organisms by the following treatment regimens: 10, 20,or 40 mg of BO3482 with 40 mg of cilastatin per kg per dose; 10, 20, or 40 mg of vancomycin per kg per dose; or 40 mg of imipenem with 40 mg of cilastatin per kg per dose. Four hours after the last treatment, the thigh muscles are removed and immediately homogenized in ice-cold 0.9% NaCl with a tissue homogenizer[2]. |
References: [1]. Adachi Y, et al. In vitro evaluation of BO-3482, a novel dithiocarbamate carbapenem with activity against methicillin-resistant staphylococci. Antimicrob Agents Chemother. 1997 Oct;41(10):2282-5. | |
| Cas No. | 198013-53-5 | SDF | |
| Canonical SMILES | OC(C1=C(SC(N(C)CCO)=S)[C@H](C)[C@@]([C@@]2([H])[C@H](O)C)([H])N1C2=O)=O.[Na] | ||
| Formula | C14H20N2NaO5S2 | M.Wt | 383.44 |
| الذوبان | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.608 mL | 13.0398 mL | 26.0797 mL |
| 5 mM | 521.6 μL | 2.608 mL | 5.2159 mL |
| 10 mM | 260.8 μL | 1.304 mL | 2.608 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)