Ac-WEHD-AFC (trifluoroacetate salt) (Synonyms: N-Acetyl-Trp-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase1 Substrate (Fluorogenic), Caspase5 Substrate (Fluorogenic))

Name: Ac-WEHD-AFC (trifluoroacetate salt)
Brand: GlpBio
SKU: GC46796
Availability: InStock
Rating: 5 (36 reviews)

رقم الكتالوجGC46796

A neuropeptide with diverse biological activities

Products are for research use only. Not for human use. We do not sell to patients.

Ac-WEHD-AFC (trifluoroacetate salt) التركيب الكيميائي

Cas No.: N/A

الحجم

السعر

المخزون

الكميّة

1 mg	117٫00	متوفر
5 mg	297٫00	متوفر
10 mg	533٫00	متوفر

Tel：(909) 407-4943 Email: sales@glpbio.com

مراجعات العميل

بناء على آراء العملاء.

Sample solution is provided at 25 µL, 10mM.

منتجات GlpBio المذكورة في الأوراق ذات السمعة الطيبة

Nature
610.7931 (2022): 366-372

Nature
618, 1017–1023 (2023)

Cell
183.7 (2020): 1867-1883

Cell Research
31, 1291–1307 (2021)

Cell Research
33, 273–287 (2023)

Cell Research
33, 546–561 (2023)

Molecular Cancer
21.1 (2022): 1-17

Molecular Cancer
21.1 (2022): 1-18

Cancer Cell
39 (2021): 1-16

Cell Host Microbe
30.8 (2022): 1124-1138

Cell Host Microbe
31.5 (2023): 766-780

Cell Host Microbe
31.3 (2023): 418-43

Cell Metabolism
34.2 (2022): 240-255

Ann Rheum Dis
82(4): 533-545 (2023)

Cell Mol Immunol
20, 264–276 (2023)

Adv Funct Mater
33.35 (2023): 2214998

Description Chemical Properties Product Documents

Product Documents

Quality Control & SDS

View current batch:

Purity: >95.00%

Ac-WEHD-AFC is a fluorogenic substrate that can be cleaved by group I caspases (caspase-1, -4, and -5).¹ Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

1.Park, K., Kuechle, M.K., Choe, Y., et al.Expression and characterization of constitutively active human caspase-14Biochemical and Biophysical Research Communications347(4)941-948(2006)

Cas No.	N/A	SDF
المرادفات	N-Acetyl-Trp-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase1 Substrate (Fluorogenic), Caspase5 Substrate (Fluorogenic)
Canonical SMILES	OC(C[C@H](NC([C@@H](NC([C@@H](NC([C@H](CC1=CNC2=C1C=CC=C2)NC(C)=O)=O)CCC(O)=O)=O)CC3=CNC=N3)=O)C(NC(C=C4O5)=CC=C4C(C(F)(F)F)=CC5=O)=O)=O.FC(F)(C(O)=O)F
Formula	C38H37F3N8O11.XCF3COOH	M.Wt	838.7
الذوبان	DMSO: 10 mg/ml,Water: 1 mg/ml	Storage	Store at -20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	1.1923 mL	5.9616 mL	11.9232 mL
	5 mM	0.2385 mL	1.1923 mL	2.3846 mL
	10 mM	0.1192 mL	0.5962 mL	1.1923 mL

حاسبة المولارية
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احسب

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal:μL

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

مراجعات

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Average Rating: 5 ★★★★★ (Based on Reviews and 36 reference(s) in Google Scholar.)

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Ac-WEHD-AFC (trifluoroacetate salt) (Synonyms: N-Acetyl-Trp-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase1 Substrate (Fluorogenic), Caspase5 Substrate (Fluorogenic))

مراجعات العميل

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