ClAP (Alkaline Phosphatase, Calf intestinal) |
رقم الكتالوجGC20061
One-Click Copy Product Info
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ClAP (Alkaline Phosphatase, Calf intestinal) is a membrane-bound glycoprotein that hydrolyzes a broad range of monophosphate esters at alkaline pH.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9001-78-9
Sample solution is provided at 25 µL, 10mM.
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ClAP (Alkaline Phosphatase, Calf intestinal) is a membrane-bound glycoprotein that hydrolyzes a broad range of monophosphate esters at alkaline pH [1]. ClAP can detoxify various inflammatory mediators such as lipopolysaccharide (LPS), lipoteichoic acid, CpG DNA, and adenosine triphosphate [2]. ClAP has been widely used to alleviate the LPS toxicity in mice and piglets, and to enhance the survival of mice after Escherichia coli infection[3].
In vivo, ClAP treatment via oral administration at a dose of 700U/kg daily for 6 days significantly improved experimental colitis in rats and reduced bacterial translocation[4]. 15 minutes after the cecal ligation and perforation surgery, a single intravenous injection of ClAP (0.15IU/g) can alleviate the inflammatory response of secondary peritonitis in mice and reduce the associated liver and lung damage[5].
References:
[1] Chirambo G M, van Niekerk C, Crowther N J. The role of alkaline phosphatase in intracellular lipid accumulation in the human hepatocarcinoma cell line, HepG2[J]. Experimental and Molecular Pathology, 2017, 102(2): 224-229.
[2] Hyoju S K, Morrison S, Gul S, et al. Intestinal alkaline phosphatase decreases intraperitoneal adhesion formation[J]. Journal of Surgical Research, 2017, 208: 84-92.
[3] Beumer C, Wulferink M, Raaben W, et al. Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets[J]. The Journal of pharmacology and experimental therapeutics, 2003, 307(2): 737-744.
[4] Martínez-Moya P, Ortega-González M, González R, et al. Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats[J]. Pharmacological research, 2012, 66(2): 144-153.
[5] van Veen S Q, van Vliet A K, Wulferink M, et al. Bovine intestinal alkaline phosphatase attenuates the inflammatory response in secondary peritonitis in mice[J]. Infection and immunity, 2005, 73(7): 4309-4314.
| Animal experiment [1]: | |
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Animal models |
Female Wistar rats |
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Preparation Method |
Female Wistar rats (175-225g) were housed in cages and maintained in air-conditioned animal quarters with a 12h light–dark cycle and free access to autoclaved tap water and a standard chow diet. rats were fasted overnight and anesthetized with halothane. Under these conditions, rats were given 10mg of trinitrobenzene sulfonic acid (TNBS) dissolved in 0.25ml of 50% ethanol (v/v) by means of a cannula inserted 8cm through the anus. Rats were kept in a head-down position for an additional 30s and returned to their cage. Rats were administered 700U/kg/day of ClAP by the oral route. Oral treatments started 1h before colitis induction. Afterward treatments were given daily until mice were sacrificed (cervical dislocation) at day 6. Food and water intake and body weight were determined daily. Colon assessment and analysis of bacterial translocation. |
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Dosage form |
700U/kg/day for 6 days; p.o. |
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Applications |
ClAP treatment inhibited improved experimental colitis in rats and reduced bacterial translocation. |
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References: |
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| Cas No. | 9001-78-9 | SDF | |
| Formula | M.Wt | ||
| الذوبان | H2O : 100 mg/mL (Need ultrasonic) | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 28000 u/ml liquid Appearance: liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)