Tetraglycine (H-Gly-Gly-Gly-Gly-OH) |
رقم الكتالوجGA22446
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Tetraglycine (H-Gly-Gly-Gly-Gly-OH) is an oligopeptide composed of four glycine monomers.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 637-84-3
Sample solution is provided at 25 µL, 10mM.
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Tetraglycine (H-Gly-Gly-Gly-Gly-OH) is an oligopeptide composed of four glycine monomers[1]. Tetraglycine can be used as a "flexible spacer/linker" in NHS ester coupling reactions[2].
In vitro, The EC50 value for the intracellular calcium ion response of NCI-H716 cells to Tetraglycine is 3mM. Application of 10mM Tetraglycine induces membrane depolarization. 15min of treatment with 10mM Tetraglycine does not affect the increase in intracellular cAMP formation. 2h of treatment with 10mM Tetraglycine promotes GLP-1 secretion from cells[3].
In vivo, Tetraglycine used as a "metabolism and transport probe". The plasma half-lives of Tetraglycine is about 5min. Tetraglycinecan be absorbed and accumulated by tissues, especially the kidneys[2].
References:
[1] Adibi SA, Morse EL. Enrichment of glycine pool in plasma and tissues by glycine, di-, tri-, and tetraglycine. Am J Physiol. 1982;243(5):E413-E417.
[2] Khan MA, Ghanim RW, Kiser MR, et al. Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers. Nanomaterials (Basel). 2022;12(4):608.
[3] Le Nevé B, Daniel H. Selected tetrapeptides lead to a GLP-1 release from the human enteroendocrine cell line NCI-H716. Regul Pept. 2011;167(1):14-20.
| Cell experiment [1]: | |
Cell lines | NCI-H716 cells |
Preparation Method | Cells were exposed to a Tetraglycine (H-Gly-Gly-Gly-Gly-OH) solution, followed by measurement of cell membrane potential using DIBAC3(5), and detection of intracellular cAMP and GLP-1 levels. |
Reaction Conditions | 10mM; 20mM; 15min; 2h |
Applications | Application of 10mM Tetraglycine induced membrane depolarization. 15min of treatment with 10mM Tetraglycine did not affect the increase in intracellular cAMP formation. 2h of treatment with 20mM Tetraglycine promoted GLP-1 secretion from cells. |
| Animal experiment [2]: | |
Animal models | Male Sprague-Dawley rats; 270-300g |
Preparation Method | The right jugular vein was injected with one of the following test solutions: 150mM NaCl, 300mM Glycine, 150mM Diglycine, 100mM Triglycine, or 75mM Tetraglycine. Plasma glutathione concentrations were measured at 2, 5, 10, 15, 20, and 30 minutes after injection. The concentrations of glutathione in the liver, muscle, and kidney were measured at 5 and 30 minutes after injection. |
Dosage form | 1pM/g; i.v. |
Applications | Two minutes after the injection of glycine, there was over a tenfold increase in plasma glycine concentration. Each increase in the number of glycine residues resulted in further reduction in the initial rise in plasma glycine concentration. The plasma half-lives of all these peptides are remarkably similar and are about 5min. They were absorbed by the organization. Injection of Triglycine and Tetraglycine resulted in greater glycine concentration in the kidney than injection of either Glycine or Diglycine. Cach increase in the number of glycine residues resulted in greater recovery of glycine peptides from the kidney. |
References: | |
| Cas No. | 637-84-3 | SDF | |
| Formula | C8H14N4O5 | M.Wt | 246.22 |
| الذوبان | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0614 mL | 20.307 mL | 40.6141 mL |
| 5 mM | 812.3 μL | 4.0614 mL | 8.1228 mL |
| 10 mM | 406.1 μL | 2.0307 mL | 4.0614 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)