TAMRA NHS ester, 5-isomer |
رقم الكتالوجGC59090
One-Click Copy Product Info
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TAMRA NHS ester, 5-isomer is an amine-reactive fluorescent labeling reagent (Ex/Em=565/580nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 321862-17-3
Sample solution is provided at 25 µL, 10mM.
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TAMRA NHS ester, 5-isomer is an amine-reactive fluorescent labeling reagent (Ex/Em=565/580nm). The NHS ester group of TAMRA NHS ester, 5-isomer can efficiently and covalently bind to primary amines (-NH₂) on biomolecules such as proteins, peptides, and nucleic acids, forming stable amide bonds, thereby covalently attaching the TAMRA fluorophore to the target molecule. TAMRA NHS ester, 5-isomer can be used for fluorescent labeling of proteins, antibodies, peptides, and nucleic acids, and is widely applied in biomedical research fields such as cell imaging, flow cytometry, immunofluorescence staining, fluorescence in situ hybridization (FISH), and fluorescence resonance energy transfer (FRET)[1-4].
References:
[1] Rosier N, Grätz L, Schihada H, et al. A Versatile Sub-Nanomolar Fluorescent Ligand Enables NanoBRET Binding Studies and Single-Molecule Microscopy at the Histamine H3 Receptor. J Med Chem. 2021 Aug 12;64(15):11695-11708.
[2] Chen H, Zhang Y, Ma X, et al. Chemically-Modified Sepharose 6B Beads for Collection of Circulating Tumor Cells. Biomolecules. 2023 Jul 3;13(7):1071.
[3] Bresinsky M, Shahraki A, Kolb P, et al. Development of Fluorescent AF64394 Analogues Enables Real-Time Binding Studies for the Orphan Class A GPCR GPR3. J Med Chem. 2023 Nov 9;66(21):15025-15041.
[4] Alexandrova VV, Anisimov MN, Zaitsev AV, et al. Theory of tip structure-dependent microtubule catastrophes and damage-induced microtubule rescues. Proc Natl Acad Sci U S A. 2022 Nov 16;119(46):e2208294119.
Method for Labeling Proteins with TAMRA NHS ester, 5-isomer[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Dissolve TAMRA NHS ester, 5-isomer in dimethyl sulfoxide (DMSO) to prepare a 5mM stock solution; prepare a 30µM solution of the target protein using 200mM NaHCO₃ buffer.
2. Labeling Reaction: Under 4°C conditions, add the DMSO solution of 5-TAMRA NHS ester to the protein solution, maintaining a molar ratio of TAMRA NHS ester, 5-isomer to protein at 8:1. The reaction continues for 12 hours.
3. Purification and Labeling Efficiency Determination: After the reaction, remove excess dye. Estimate the degree of labeling by measuring the absorbance ratio (A₅₄₁/A₂₈₀) of the labeled product at 541nm and 280nm. This ratio typically corresponds to labeling 3.2 to 3.8 moles of TAMRA per mole of protein.
Precautions:
(1) The labeling reaction should be performed at 4°C to ensure protein stability and control the reaction rate.
(2) Labeling efficiency is determined by spectrophotometry; ensure the spectrophotometer is accurately calibrated.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Sokolov AV, Isakova-Sivak IN, Mezhenskaya DA, et al. Molecular mimicry of the receptor-binding domain of the SARS-CoV-2 spike protein: from the interaction of spike-specific antibodies with transferrin and lactoferrin to the antiviral effects of human recombinant lactoferrin. Biometals. 2023 Jun;36(3):437-462.
| Cas No. | 321862-17-3 | SDF | |
| Formula | C29H25N3O7 | M.Wt | 527.53 |
| الذوبان | good in DMF, DMSO, low in water | Storage | Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8956 mL | 9.4781 mL | 18.9563 mL |
| 5 mM | 379.1 μL | 1.8956 mL | 3.7913 mL |
| 10 mM | 189.6 μL | 947.8 μL | 1.8956 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)