Neochlorogenic acid methyl ester (Synonyms: 5-O-Caffeoylquinic acid methyl ester, Methyl 3-caffeoylquinate, GIP (1-39) acetate(725474-97-5 Free base), Methyl neochlorogenate) |
رقم الكتالوجGF05704
One-Click Copy Product Info
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Neochlorogenic acid methyl ester is a compound with anti-HBV activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 123410-65-1
Sample solution is provided at 25 µL, 10mM.
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Neochlorogenic acid methyl ester is a compound with anti-HBV activity[1]. Neochlorogenic acid methyl ester can be isolated from the fruit of wild eggplant and the stem of Akebia trifoliata[2][3]. In addition,Neochlorogenic acid methyl ester also demonstrated antioxidant activity and quinone reductase activity, with the concentration inducing quinone reductase activity being 6.7μM[4]. Neochlorogenic acid methyl ester is usually used in anti-HBV and antioxidant related research[5-7].
In vitro, treatment of HepG 2.2.15 cells with Neochlorogenic acid methyl ester(144.7μM) dramatically decreased the activity against HBV DNA replication, reduced secretions of HBsAg and HBeAg without cellular toxicity[7].
References:
[1] Ge, L., Wan, H., Tang, S., Chen, H., Li, J., Zhang, K., Zhou, B., Fei, J., Wu, S., & Zeng, X. (2018). Novel caffeoylquinic acid derivatives from Lonicera japonica Thunb. flower buds exert pronounced anti-HBV activities. RSC advances, 8(62), 35374–35385.
[2] Ma, C., Whitaker, B. D., & Kennelly, E. J. (2010). New 5-O-caffeoylquinic acid derivatives in fruit of the wild eggplant relative Solanum viarum. Journal of agricultural and food chemistry, 58(20), 11036–11042.
[3] Wang, J., Xu, Q. L., Zhou, Z. Y., & Tan, J. W. (2014).[Caffeoylquinic acid derivatives from stems of Akebia trifoliata].Journal of Chinese medicinal materials, 37(7), 1190–1193.
[4] Li, J., Deng, Y., Yuan, C., Pan, L., Chai, H., Keller, W. J., & Kinghorn, A. D. (2012). Antioxidant and quinone reductase-inducing constituents of black chokeberry (Aronia melanocarpa) fruits. Journal of agricultural and food chemistry, 60(46), 11551–11559.
[5] Retamozo MH, Silva CC, Tamayose CI, Carvalho JCS, Romoff P, Fávero OA, Ferreira MJP. Chemical Constituents from Leaves of Baccharis sphenophylla (Asteraceae) and Their Antioxidant Effects. Plants (Basel). 2023 Mar 10;12(6):1262. doi: 10.3390/plants12061262.
[6] Kim WR, Kim EO, Kang K, Oidovsambuu S, Jung SH, Kim BS, Nho CW, Um BH. Antioxidant activity of phenolics in leaves of three red pepper (Capsicum annuum) cultivars. J Agric Food Chem. 2014 Jan 29;62(4):850-9.
[7] Zhao Y, Geng CA, Ma YB, Huang XY, Chen H, Cao TW, He K, Wang H, Zhang XM, Chen JJ. UFLC/MS-IT-TOF guided isolation of anti-HBV active chlorogenic acid analogues from Artemisia capillaris as a traditional Chinese herb for the treatment of hepatitis. J Ethnopharmacol. 2014 Oct 28;156:147-54.
| Cell experiment [1]: | |
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Cell lines |
HepG 2.2.15 cell line |
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Preparation Method |
HepG 2.2.15 cells were cultured in RPMI–1640 medium supplemented with 10% foetal calf serum, 100mg/mL G148, 100 IU/mL penicillin, 100IU/mL streptomycin, and maintained at 37°C in a moist atmosphere containing 5% CO2. Anti-HBV procedure was carried according to previous report. The anti-HBV antigen secretion activities and cytotoxicity were assayed by ELISA and a modified MTT method, inhibition of HBV DNA replication was monitored by the real-time quantitative PCR method. Neochlorogenic acid methyl ester(144.7, 312.5, 2488.4, 2916.2nM; 72h) were dissolved in DMSO (solvent control) for anti-HBV activity and cytotoxicity assay. The final concentration of DMSO in the culture did not exceed 2.5μL/mL and did not affect the growth of cells. |
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Reaction Conditions |
144.7, 312.5, 2488.4, 2916.2nM; 72h |
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Applications |
Neochlorogenic acid methyl ester dramatically decreased the activity against HBV DNA replication, reduced secretions of HBsAg and HBeAg without cellular toxicity. |
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References: |
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| Cas No. | 123410-65-1 | SDF | |
| المرادفات | 5-O-Caffeoylquinic acid methyl ester, Methyl 3-caffeoylquinate, GIP (1-39) acetate(725474-97-5 Free base), Methyl neochlorogenate | ||
| Formula | C17H20O9 | M.Wt | 368.34 |
| الذوبان | DMSO: 55 mg/mL (149.32 mM) | Storage | 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7149 mL | 13.5744 mL | 27.1488 mL |
| 5 mM | 543 μL | 2.7149 mL | 5.4298 mL |
| 10 mM | 271.5 μL | 1.3574 mL | 2.7149 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)