Histatin 5 |
رقم الكتالوجGC31898
One-Click Copy Product Info
|
يثبط الهستاتين 5 نشاط البروتينات المعدنية لمصفوفة المضيف MMP-2 و MMP-9 مع IC50s من 0.57 و 0.25 ميكرومتر ، على التوالي
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 115966-68-2
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Histatin 5 suppresses the activities of matrix metalloproteinases MMP-2 and MMP-9 with IC50 values of 0.57 and 0.25μM, respectively[1]. Histatin 5 significantly inhibited the growth of a variety of Candida species by binding to Candida cell wall proteins (Ssa1/2) and glycans with MIC50 values of 10-20μg/ml[2]. Histatin 5 has been widely used in metal ion binding studies and antifungal studies[3].
In vitro, Histatin 5 at a concentration of 3200μg/mL for 24 hours was significantly cytotoxic to human gingival fibroblasts and dramatically inhibited cell viability[4]. Treatment of human gingival fibroblasts with 10μg/ml Histatin 5 for 20 minutes inhibited the production of inflammatory cytokines (IL-6 and IL-8) induced by P. gingivalis and lipopolysaccharides[5].
In vivo, Histatin 5 gel at a dose of 100μg/mL three times daily applied to the tongues of mice for three days prevented the occurrence of oral ulcers and significantly inhibited Candida albicans[6]. Administration of Histatin 5 (80μM) to the cornea three times daily for one day promoted wound healing in a mouse corneal injury model[7].
References:
[1] Gusman H, Travis J, Helmerhorst E J, et al. Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease[J]. Infection and immunity, 2001, 69(3): 1402-1408.
[2] Puri S, Edgerton M. How does it kill?: understanding the candidacidal mechanism of salivary histatin 5[J]. Eukaryotic cell, 2014, 13(8): 958-964.
[3] Zolin G V S, Fonseca F H, Zambom C R, et al. Histatin 5 metallopeptides and their potential against Candida albicans pathogenicity and drug resistance[J]. Biomolecules, 2021, 11(8): 1209.
[4] Moffa E B, Mussi M C M, Xiao Y, et al. Histatin 5 inhibits adhesion of C. albicans to reconstructed human oral epithelium[J]. Frontiers in Microbiology, 2015, 6: 885.
[5] Imatani T, Kato T, Minaguchi K, et al. Histatin 5 inhibits inflammatory cytokine induction from human gingival fibroblasts by Porphyromonas gingivalis[J]. Oral microbiology and immunology, 2000, 15(6): 378-382.
[6] Kong E F, Tsui C, Boyce H, et al. Development and in vivo evaluation of a novel histatin-5 bioadhesive hydrogel formulation against oral candidiasis[J]. Antimicrobial agents and chemotherapy, 2016, 60(2): 881-889.
[7] Shah D, Son K N, Kalmodia S, et al. Wound healing properties of histatin-5 and identification of a functional domain required for histatin-5-induced cell migration[J]. Molecular Therapy Methods & Clinical Development, 2020, 17: 709-716.
| Cell experiment [1]: | |
Cell lines | Human gingival fibroblast cells |
Preparation Method | The cytotoxic effect of Histatin 5 was assessed on human gingival fibroblast cells grown in Dulbeccco's modified Eagle medium supplemented with antibiotic-fungal solution and 10% v/v fetal bovine serum. The incubation temperature was 37°C with 5% CO2 in air and 95% air concentration. Cells were cultured to confluence (90%) and removed with trypsin (0.05%)/EDTA (0.02%) in 1×PBS. Medium was added to inactivate trypsin, and cells were then centrifuged at 2000rpm for 5min, resuspended, and re-seeded. The medium was changed two to three times a week. Total viable cell counts were performed in an incubator. Cell suspensions containing 2.0×104 cells/ml were incubated in 24-well plates for 48 hours at 37°C in a humidified environment with 5% CO2. At the end of the culture, the medium was discarded, and adherent cells remained at the bottom of the plate. Gradient dilutions of Histatin 5 (100, 200, 400, 800, 1600, 3200, 6400, and 12800μg/mL) were performed with fresh medium. The plates were incubated at 37°C in an atmosphere of 5% CO2 and 95% air for 24 hours. MTT assay was used to measure mitochondrial dehydrogenase activity. After the cells were grown in the control or test medium for 24h, 100μL of MTT stock solution was added to each well. The culture plates were incubated at 37°C in 5% CO2 for 4 hours. At the end of incubation, the cultures were removed from the incubator and 100μL of MTT solubilization solution was added to dissolve the resulting formazan crystals. The plates were then shaken until the crystals were completely dissolved, and the absorbance was measured at a wavelength of 570nm by spectrophotometry; all experiments were performed three times. |
Reaction Conditions | 100, 200, 400, 800, 1600, 3200, 6400, and 12800μg/mL; 24h |
Applications | Histatin 5 at concentrations above 3200μg/mL was significantly cytotoxic, resulting in an apparent reduction in gingival fibroblast cell viability. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | The C57BL/6J mice aged between twelve and nineteen weeks received anesthesia through an intraperitoneal injection containing ketamine at 100mg/kg and xylazine at 5mg/kg. A circular 2.0-mm central epithelial segment was delineated with a 2-mm biopsy punch following 2 drops of topical 0.5% proparacaine, then removed with a brush. Histatin 5 (80μM), SHRGY (80μM) was applied to the cornea three times a day during the treatment (n = 7) or the control (n = 7) group. At 0, 18, and 24h, corneas were stained with fluorescein and imaged using a photo-slit lamp with a camera. The remaining wound areas were measured with the ImageJ software at each indicated time point and were compared with the baseline wound area for each mouse. The percentage of the remaining wound area was calculated for each time point. |
Dosage form | 80μM for three times a day; apply to the cornea |
Applications | Histatin 5 treatment significantly promoted wound healing in a mouse corneal injury model. |
References: | |
| Cas No. | 115966-68-2 | SDF | |
| Canonical SMILES | Asp-Ser-His-Ala-Lys-Arg-His-His-Gly-Tyr-Lys-Arg-Lys-Phe-His-Glu-Lys-His-His-Ser-His-Arg-Gly-Tyr | ||
| Formula | C133H195N51O33 | M.Wt | 3036.29 |
| الذوبان | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 329.3 μL | 1.6467 mL | 3.2935 mL |
| 5 mM | 65.9 μL | 329.3 μL | 658.7 μL |
| 10 mM | 32.9 μL | 164.7 μL | 329.3 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)