HT-2 Toxin (Synonyms: NSC 278571) |
رقم الكتالوجGC18185
One-Click Copy Product Info
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HT-2 Toxin هو مستقلب نشط ، منزوع الأسيتات من التوكسين T-2
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 26934-87-2
Sample solution is provided at 25 µL, 10mM.
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HT-2 Toxin, a triterpenoid fungal metabolite of T-2 toxin, inhibits protein synthesis in eukaryotic cells, resulting in apoptosis, immunosuppression, and tissue damage [1-3].
In porcine brain capillary endothelial cells, the cell viability of cells incubated with HT-2 Toxin (1nM-10µM; 24h, 48h) decreased in a concentration-dependent manner [4]. In porcine oocytes, exposure to HT-2 Toxin (10nM, 50nM, 100nM; 44h) causes oxidative stress induced apoptosis/autophagy [5]. In human chondrocytes, exposure to HT-2 Toxin (20ng/mL; 48h) significantly reduced chondrocyte viability [6]. In porcine oocytes, HT-2 Toxin (100nM; 48h) impairs porcine oocyte in vitro maturation through disruption of endomembrane system [7].
In C57BL/6J mice, the body weight of mice exposed to HT-2 Toxin (1.6mg/kg, 3.2mg/kg; ig; 4 weeks) decreased in a dose-dependent manner [8]. In rat, HT-2 Toxin (0.01mg/kg, 0.1mg/kg, 0.5mg/kg, 1mg/kg; ip; 96h) elicited anorectic effects of varying durations in a dose-dependent manner [9].
References:
[1]. Nathanail AV, Varga E, Meng-Reiterer J, et al. Metabolism of the Fusarium mycotoxins T-2 toxin and HT-2 toxin in wheat. Journal of agricultural and food chemistry. 2015 Sep 9; 63(35): 7862-7872.
[2]. Meneely J, Greer B, Kolawole O, et al. T-2 and HT-2 toxins: toxicity, occurrence and analysis: a review. Toxins. 2023 Jul 29; 15(8): 481.
[3]. Zhu CC, Zhang Y, Duan X, et al. Toxic effects of HT-2 toxin on mouse oocytes and its possible mechanisms. Archives of toxicology. 2016 Jun; 90: 1495-1505.
[4]. Weidner M, Hüwel S, Ebert F, et al. Influence of T-2 and HT-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects. PloS one. 2013 Mar 27; 8(3): e60484.
[5]. Zhang Y, Han J, Zhu CC, et al. Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes. Scientific reports. 2016 Sep 23; 6(1): 33904.
[6]. Yu FF, Lin XL, Wang X, et al Comparison of apoptosis and autophagy in human chondrocytes induced by the T-2 and HT-2 toxins. Toxins. 2019 May 8; 11(5): 260.
[7]. Li JR, Wu SL, Hu LL, et al. HT-2 toxin impairs porcine oocyte in vitro maturation through disruption of endomembrane system. Theriogenology. 2024 Sep 15; 226: 286-293.
[8]. Hao S, Yao C, Meng P, et al. The spinal consequences of HT-2 toxin and selenium deficiency during bone maturation in mice. Mycotoxin research. 2025 Feb; 41(1): 77-91.
[9]. Zhang J, Zhang H, Liu S, et al. Comparison of anorectic potencies of type A trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and neosolaniol. Toxins. 2018 Apr 29; 10(5): 179.
| Cell experiment [1]: | |
Cell lines | Porcine brain capillary endothelial cells |
Preparation Method | Porcine brain capillary endothelial cells were seeded on Collagen-G-coated 96-well plates (250,000cells/cm2) and cultured according to the treatment on Transwell® filter systems (DIV 2: plating medium replaced by serum-free medium on DIV 4 for 2 days). Incubation with various T-2 and HT-2 Toxin concentrations (1nM-10µM) was carried out over 24h and 48h following the addition of the WST-8 solution [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] and incubation according to the manufacturer's manual. |
Reaction Conditions | 1nM-10µM; 24h, 48h |
Applications | The cell viability of cells incubated with HT-2 Toxin decreased in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | Male C57BL/6J mice were subjected to subchronic toxin exposure. A total of 48 mice were divided into six groups, based on toxin concentrations determined in preliminary studies. The groups included a control group, a low selenium diet group, two groups exposed to different concentrations of HT-2 Toxin (1.6mg/kg and 3.2mg/kg), and two groups combining a low selenium diet with each of these HT-2 Toxin dosages. Over a 4-week period, all mice received their designated treatments via oral gavage, mimicking typical clinical and environmental exposure scenarios. |
Dosage form | 1.6mg/kg, 3.2mg/kg; ig; 4 weeks |
Applications | The body weight of mice exposed to HT-2 Toxin decreased in a dose-dependent manner. |
References: | |
| Cas No. | 26934-87-2 | SDF | |
| المرادفات | NSC 278571 | ||
| Chemical Name | 12,13-epoxy-trichothec-9-ene-3α,4β,8α,15-tetrol, 15-acetate 8-(3-methylbutanoate) | ||
| Canonical SMILES | CC1=C[C@]2([H])[C@]([C@]([C@H](O)[C@H]3O)(C)[C@@]4(OC4)[C@]3([H])O2)(COC(C)=O)C[C@@H]1OC(CC(C)C)=O | ||
| Formula | C22H32O8 | M.Wt | 424.5 |
| الذوبان | Dichloromethane: 5 mg/ml,DMSO: soluble,Ethanol: soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3557 mL | 11.7786 mL | 23.5571 mL |
| 5 mM | 471.1 μL | 2.3557 mL | 4.7114 mL |
| 10 mM | 235.6 μL | 1.1779 mL | 2.3557 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)