الصفحة الرئيسية>>Signaling Pathways>> Stem Cell>> Smoothened>>Jervine

Jervine (Synonyms: NSC 7520, NSC 23898)

رقم الكتالوجGC11111

Jervine (11-Ketocyclopamine) هو مثبط قوي للقنفذ (Hh) مع IC50 من 500-700 نانومتر

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Jervine التركيب الكيميائي

Cas No.: 469-59-0

الحجم السعر المخزون الكميّة
1mg
25٫00
متوفر
5mg
57٫00
متوفر
10mg
105٫00
متوفر
25mg
221٫00
متوفر
50mg
309٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Description of Jervine

Jervine(11-Ketocyclopamine) is a naturally occuring steroidal alkaloid that causes cyclopia by blocking sonic hedgehog(Shh) signaling; Jervine is an inhibitor of Smo.IC50 value:Target: sonic hedgehog is derived from the Veratrum plant species. It is a close structural analog of cyclopamine which specifically inhibits the hedgehog (Hh) pathway by interaction with the hedgehog signaling protein Smo. Jervine can be used to induce abnormal morphogenesis in a number of experimental models. Jervine is an inhibitor of Smo.

References:
[1]. Incardona JP, et al. Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport. Dev Biol. 2000 Aug 15;224(2):440-52.
[2]. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.
[3]. Mistretta CM, et al. Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium. Dev Biol. 2003 Feb 1;254(1):1-18.

Chemical Properties of Jervine

Cas No. 469-59-0 SDF
المرادفات NSC 7520, NSC 23898
Chemical Name (3S,3'R,3'aS,6'S,6aS,6bS,7'aR,9R,11aS,11bR)-3-hydroxy-3',6',10,11b-tetramethylspiro[1,2,3,4,6,6a,6b,7,8,11a-decahydrobenzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-11-one
Canonical SMILES CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5C(=O)C4=C3C)C)O)C)NC1
Formula C27H39NO3 M.Wt 425.6
الذوبان DMF: 2 mg/ml,Ethanol: 10 mg/ml,Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Jervine

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.3496 mL 11.7481 mL 23.4962 mL
5 mM 0.4699 mL 2.3496 mL 4.6992 mL
10 mM 0.235 mL 1.1748 mL 2.3496 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 16 reference(s) in Google Scholar.)

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