N-3-hydroxybutyryl-L-Homoserine lactone (Synonyms: 3-OH-C4-HSL, N-3-hydroxybutanoyl-L-Homoserine lactone, N-3-hydroxybutyryl-L-HSL, N-3-hydroxybutanoyl-L-HSL) |
| رقم الكتالوجGC46181 |
N-3-hydroxybutyryl-L-Homoserine lactone is an N-acyl-homoserine lactone produced by the bacterium V. splendidus and has been found in sequencing batch biofilm reactors used in wastewater treatment.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1325550-06-8
Sample solution is provided at 25 µL, 10mM.
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N-3-hydroxybutyryl-L-Homoserine lactone is an N-acyl-homoserine lactone produced by the bacterium V. splendidus and has been found in sequencing batch biofilm reactors used in wastewater treatment[1][2]. N-3-hydroxybutyryl-L-Homoserine lactone is a signaling molecule that is involved in bacterial quorum sensing (QS)[3]. N-3-hydroxybutyryl-L-Homoserine lactone senses the density of bacterial population, regulates the cellular process, such as bioluminescence, biofilm formation, and exo-enzyme production[4][5]. N-3-hydroxybutyryl-L-homoserine lactone can be used for screening antimicrobial drugs that interfere with quorum sensing and for studying bacterial infection mechanisms[6][7].
References:
[1] Purohit AA, Johansen JA, Hansen H, et al. Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family. J Appl Microbiol. 2013;115(3):835-847.
[2] Feng Z, Sun Y, Li T, Meng F, Wu G. Operational pattern affects nitritation, microbial community and quorum sensing in nitrifying wastewater treatment systems. Sci Total Environ. 2019;677:456-465.
[3] Ke X, Miller LC, Bassler BL. Determinants governing ligand specificity of the Vibrio harveyi LuxN quorum-sensing receptor. Mol Microbiol. 2015;95(1):127-142.
[4] Rajamani S, Sayre RT. A sensitive fluorescence reporter for monitoring quorum sensing regulated protease production in Vibrio harveyi. J Microbiol Methods. 2011;84(2):189-193.
[5] Yan G, Fu L, Ming H, Chen C, Zhou D. Exploring an Efficient and Eco-Friendly Signaling Molecule and Its Quorum Quenching Ability for Controlling Microcystis Blooms. Environ Sci Technol. 2023;57(44):16929-16939.
[6] Hanzelka BL, Parsek MR, Val DL, Dunlap PV, Cronan JE Jr, Greenberg EP. Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein. J Bacteriol. 1999;181(18):5766-5770.
[7] Sharma N, Pandey S, Yadav M, et al. Biomolecular map of albumin identifies signatures of severity and early mortality in acute liver failure. J Hepatol. 2023;79(3):677-691.
| Cas No. | 1325550-06-8 | SDF | |
| المرادفات | 3-OH-C4-HSL, N-3-hydroxybutanoyl-L-Homoserine lactone, N-3-hydroxybutyryl-L-HSL, N-3-hydroxybutanoyl-L-HSL | ||
| Canonical SMILES | OC(C)CC(N[C@@H]1C(OCC1)=O)=O | ||
| Formula | C8H13NO4 | M.Wt | 187.2 |
| الذوبان | DMF: soluble; DMSO: soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 5.3419 mL | 26.7094 mL | 53.4188 mL |
| 5 mM | 1.0684 mL | 5.3419 mL | 10.6838 mL |
| 10 mM | 534.2 μL | 2.6709 mL | 5.3419 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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