O4I1 |
رقم الكتالوجGC12499 |
O4I1 هو محفز قوي Oct3 / 4
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 175135-47-4
Sample solution is provided at 25 µL, 10mM.
O4I1 is a potent Oct3/4 inducer implicated in promoting the expression and stabilization of Oct3/4. O4I1 can enhance the transcriptional activity of Oct3/4 in a variety of human somatic cells. Oct3/4, also known as POU5F1 and Oct4, is a transcription factor which is essential for self-renewal and pluripotency of embryonic stem cells. Oct3/4 is specifically expressed in the ES cells, epiblast, preimplantation embryos, and germ cells, et al [1].
When treated the synthesized cDNA from RNA isolated from the HEK293 cells with 10 µM and 20 µM O4I1 for 72 hrs, the Oct3/4 mRNA levels increased about 2.5- and 4-fold respectively. In human adult primary fibroblast (HF) and human neonatal foreskin fibroblast (HFF) cell lines, treatment with 10 µM O4I1 increased the gene and protein expression level of Oct3/4 after 72 hrs incubation in HF cells and 7 days in HFF cells. The derivatives of O4I1 such as compound 2, showed a time-dependent activation of Oct3/4 and markedly elevated the efficiency of Oct3/4 induction [1].
Reference:
[1].Cheng X, Dimou E, Alborzinia H, et al. Identification of 2-[4-[(4-Methoxyphenyl) methoxy]-phenyl] acetonitrile and Derivatives as Potent Oct3/4 Inducers[J]. Journal of medicinal chemistry, 2015, 58(12): 4976-4983.
Cas No. | 175135-47-4 | SDF | |
Chemical Name | 2-(4-((4-methoxybenzyl)oxy)phenyl)acetonitrile | ||
Canonical SMILES | COC1=CC=C(COC2=CC=C(CC#N)C=C2)C=C1 | ||
Formula | C16H15NO2 | M.Wt | 253.3 |
الذوبان | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2)(1:5): 0.16 mg/ml,Ethanol: slightly | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
1 mM | 3.9479 mL | 19.7394 mL | 39.4789 mL |
5 mM | 0.7896 mL | 3.9479 mL | 7.8958 mL |
10 mM | 0.3948 mL | 1.9739 mL | 3.9479 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5
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