PL553 |
| رقم الكتالوجGC30662 |
PL553 عبارة عن ركيزة فلورية محددة وعالية التقارب من Leukotriene A4 hydrolase ، مع Λmax 210 نانومتر و Λem من 410 نانومتر
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1456872-74-4
Sample solution is provided at 25 µL, 10mM.
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PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.
PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1].
[1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.
Kinase experiment: | The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1]. |
References: [1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61. | |
| Cas No. | 1456872-74-4 | SDF | |
| Canonical SMILES | O=C(NC1=CC=C2C=CC=CC2=C1)[C@@H](N)CC3=CC=C(C(C4=CC=CC=C4)=O)C=C3.O=C(O)C(F)(F)F | ||
| Formula | C28H23F3N2O4 | M.Wt | 508.49 |
| الذوبان | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9666 mL | 9.833 mL | 19.6661 mL |
| 5 mM | 393.3 μL | 1.9666 mL | 3.9332 mL |
| 10 mM | 196.7 μL | 983.3 μL | 1.9666 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)
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