Sulforhodamine 101 (Synonyms: SR 101, Sulforhodamine 640) |
رقم الكتالوجGC12347
One-Click Copy Product Info
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Sulforhodamine 101 (SR101) هو صبغة فلورية حمراء مذبذبة (الإثارة / الانبعاث: 586/605 نانومتر)
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 60311-02-6
Sample solution is provided at 25 µL, 10mM.
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Sulforhodamine 101 is a red fluorescent dye.
Sulforhodamine 101 does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].
In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Sulforhodamine 101 or topical application of 100 μM[1].
References:
[1]. Hülsmann S, et al. Limitations of Sulforhodamine 101 for Brain Imaging. Front Cell Neurosci. 2017 Feb 28;11:44.
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Cell experiment: |
Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Sulforhodamine 101 for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier[1]. |
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Animal experiment: |
For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has been reported to be successful[1]. |
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References: [1]. Hülsmann S, et al. Limitations of Sulforhodamine 101 for Brain Imaging. Front Cell Neurosci. 2017 Feb 28;11:44. |
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| Cas No. | 60311-02-6 | SDF | |
| المرادفات | SR 101, Sulforhodamine 640 | ||
| Chemical Name | A name could not be generated for this structure. | ||
| Canonical SMILES | [O-]S(=O)(C1=CC(S(O)(=O)=O)=CC=C1C2=C3C(OC(C2=C4)=C5C6=C4CCCN6CCC5)=C7C(C8=C3)=[N+](CCC8)CCC7)=O | ||
| Formula | C31H30N2O7S2 | M.Wt | 606.71 |
| الذوبان | DMF: 20 mg/ml,DMSO: 25 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): 2 mg/ml | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6482 mL | 8.2412 mL | 16.4823 mL |
| 5 mM | 329.6 μL | 1.6482 mL | 3.2965 mL |
| 10 mM | 164.8 μL | 824.1 μL | 1.6482 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)