Trx-red (Synonyms: NBL-SS perchlorate) |
| رقم الكتالوجGC61590 |
Trx-red (NBL-SS perchlorate) هو مسبار فلورسنت ينبعث منه اللون الأحمر مشتق من فلوروفور النيل الأزرق
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Cas No.: 2368978-96-3
Sample solution is provided at 25 µL, 10mM.
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Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (Λex=615 nm, Λem=661 nm)[1].
Trx-red (NBL-SS) displayed multiple favorable properties, such as red emission, fast response, and high fluorescence signal. Thioredoxin (Trx) is a ubiquitous redox-regulating protein essentially involved in cell growth, differentiation, and death[1]. When HeLa cells are incubated with Trx-red (NBL-SS; 5 μM; 0-30 min), a clear time-dependent increase of the fluorescence is observed[1].
Trx-red (NBL-SS; 10 μM; 30 min) is also suitable to image thioredoxin in 6 day-old zebrafish larvae[1].
[1]. Huiyi Jia, et al. Fluorophore-Dependent Cleavage of Disulfide Bond Leading to a Highly Selective Fluorescent Probe of Thioredoxin. Anal Chem. 2019 Jul 2;91(13):8524-8531.
| Cas No. | 2368978-96-3 | SDF | |
| المرادفات | NBL-SS perchlorate | ||
| Canonical SMILES | O=C(NC1=CC(OC2=C/3)=C(C4=C1C=CC=C4)N=C2C=CC3=[N+](CCC(OC)=O)/CCC(OC)=O)OCCSSCCO.O=Cl(=O)([O-])=O | ||
| Formula | C29H32ClN3O12S2 | M.Wt | 714.16 |
| الذوبان | DMSO : 50 mg/mL (70.01 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.4002 mL | 7.0012 mL | 14.0025 mL |
| 5 mM | 280 μL | 1.4002 mL | 2.8005 mL |
| 10 mM | 140 μL | 700.1 μL | 1.4002 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)
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