BTB06584 (Synonyms: BTB) |
| رقم الكتالوجGC16833 |
BTB06584 هو مثبط انتقائي يعتمد على الميتوكوندريا F1Fo-ATPase ويعتمد على IF1 دون المساومة على تخليق ATPيمكن أن يؤخر BTB06584 موت الخلايا الإقفارية
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Cas No.: 219793-45-0
Sample solution is provided at 25 µL, 10mM.
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Ischaemia compromises mitochondrial respiration. Consequently, the mitochondrial 1Fo-ATP synthase reverses and plays as a proton-pumping ATPase, so maintaining the mitochondrial membrane potential (ΔΨm), while accelerating ATP depletion and cell death. BTB06584 is an IF1-dependent selective inhibitor of the mitochondrial F1Fo-ATPase.
In vitro: BTB inhibited F1Fo-ATPase activity with no effect on the mitochondrial membrane potential (ΔΨm) or O2 consumption. ATP consumption decreased via inhibition of respiration, and ischaemic cell death was reduced. BTB efficiency increased by IF1 overexpression and reduced by silencing this protein. In addition, BTB rescued defective haemoglobin synthesis in zebrafish pinotage (pnt) mutants in which expression of the Atpif1a gene is lost [1].
In vivo: The BTB-mediated protection was further tested in neurons. Primary cultured cortical neurons of mice were exposed to OGD, treated or not with BTB, followed by RX. The resulting cell death was significantly reduced by BTB, as scored by PI staining, compared with cells left untreated during OGD [1].
Clinical trials: Currenlty no clinical data are available.
Reference:
[1] Ivanes F, Faccenda D, Gatliff J, Ahmed AA, Cocco S, Cheng CH, Allan E, Russell C, Duchen MR, Campanella M. The compound BTB06584 is an IF1-dependent selective inhibitor of the mitochondrial F1 Fo-ATPase. Br J Pharmacol. 2014;171(18):4193-206.
| Cas No. | 219793-45-0 | SDF | |
| المرادفات | BTB | ||
| Chemical Name | [5-(benzenesulfonyl)-2-nitrophenyl] 4-chlorobenzoate | ||
| Canonical SMILES | C1=CC=C(C=C1)S(=O)(=O)C2=CC(=C(C=C2)[N+](=O)[O-])OC(=O)C3=CC=C(C=C3)Cl | ||
| Formula | C19H12ClNO6S | M.Wt | 417.82 |
| الذوبان | ≥ 17.05mg/mL in DMSO | Storage | Store at -20° C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3934 mL | 11.9669 mL | 23.9338 mL |
| 5 mM | 0.4787 mL | 2.3934 mL | 4.7868 mL |
| 10 mM | 0.2393 mL | 1.1967 mL | 2.3934 mL |
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Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)
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