C16 Ceramide (d18:1/16:0) (Synonyms: Palmitoyl Ceramide)

C16 ceramide (d18:1/16:0), as an endogenous ceramide, generated by ceramide synthase 6 (CerS6), that acts as a lipid second messenger to regulate apoptosis and stress signaling[1]. C16-ceramide plays a pivotal role in inducing insulin resistance[2].

In vitro, treatment with 100 µM synthetic C16 ceramide (d18:1/16:0), or accumulation of C16 ceramide (d18:1/16:0) through PPMP (30 µM) (namely, a selective inhibitor of glucosylceramide synthase) or MAPP (50 µM) (namely, a specific ceramidase inhibitor) induces apoptosis in neutrophil cultures via caspase-3 activation[3]. In vitro, 12 µM C16-ceramide treatment in HCT116 cells induces EMD (emerin) phosphorylation[4]. In vitro, 1 µM C16 ceramide partially rescued the LASP1-actin (play a role in cell migration) interaction under a CERS6 silencing condition[5]. Exogenous C16-ceramide (20 µM) and acid sphingomyelinase induced trophoblast apoptosis, an effect abrogated completely by cotreatment with 10 ng/ml EGF(epidermal growth factor)[6].

References:
[1]White-Gilbertson S, et al. Ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells. Oncogene. 2009 Feb 26;28(8):1132-41.
[2]Chathoth S, et al. Insulin resistance induced by de novo pathway-generated C16-ceramide is associated with type 2 diabetes in an obese population. Lipids Health Dis. 2022 Feb 20;21(1):24.
[3]Seumois G, et al. De novo C16- and C24-ceramide generation contributes to spontaneous neutrophil apoptosis. J Leukoc Biol. 2007 Jun;81(6):1477-86.
[4]Deroyer C, et al. New role for EMD (emerin), a key inner nuclear membrane protein, as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway. Autophagy. 2014 Jul;10(7):1229-40.
[5]Payne SG, et al. Epidermal growth factor inhibits ceramide-induced apoptosis and lowers ceramide levels in primary placental trophoblasts. J Cell Physiol. 1999 Aug;180(2):263-70.