C16 Ceramide (d18:1/16:0) (Synonyms: Palmitoyl Ceramide) |
Catalog No.GC43028
One-Click Copy Product Info
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C16 ceramide (d18:1/16:0), as an endogenous ceramide, generated by ceramide synthase 6 (CerS6), that acts as a lipid second messenger to regulate apoptosis and stress signaling[1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 24696-26-2
Sample solution is provided at 25 µL, 10mM.
C16 ceramide (d18:1/16:0), as an endogenous ceramide, generated by ceramide synthase 6 (CerS6), that acts as a lipid second messenger to regulate apoptosis and stress signaling[1]. C16-ceramide plays a pivotal role in inducing insulin resistance[2].
In vitro, treatment with 100 µM synthetic C16 ceramide (d18:1/16:0), or accumulation of C16 ceramide (d18:1/16:0) through PPMP (30 µM) (namely, a selective inhibitor of glucosylceramide synthase) or MAPP (50 µM) (namely, a specific ceramidase inhibitor) induces apoptosis in neutrophil cultures via caspase-3 activation[3]. In vitro, 12 µM C16-ceramide treatment in HCT116 cells induces EMD (emerin) phosphorylation[4]. In vitro, 1 µM C16 ceramide partially rescued the LASP1-actin (play a role in cell migration) interaction under a CERS6 silencing condition[5]. Exogenous C16-ceramide (20 µM) and acid sphingomyelinase induced trophoblast apoptosis, an effect abrogated completely by cotreatment with 10 ng/ml EGF(epidermal growth factor)[6].
References:
[1]White-Gilbertson S, et al. Ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells. Oncogene. 2009 Feb 26;28(8):1132-41.
[2]Chathoth S, et al. Insulin resistance induced by de novo pathway-generated C16-ceramide is associated with type 2 diabetes in an obese population. Lipids Health Dis. 2022 Feb 20;21(1):24.
[3]Seumois G, et al. De novo C16- and C24-ceramide generation contributes to spontaneous neutrophil apoptosis. J Leukoc Biol. 2007 Jun;81(6):1477-86.
[4]Deroyer C, et al. New role for EMD (emerin), a key inner nuclear membrane protein, as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway. Autophagy. 2014 Jul;10(7):1229-40.
[5]Payne SG, et al. Epidermal growth factor inhibits ceramide-induced apoptosis and lowers ceramide levels in primary placental trophoblasts. J Cell Physiol. 1999 Aug;180(2):263-70.
| Cell experiment [1]: | |
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Cell lines |
SW620 cells |
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Preparation Method |
Exogenous C16 ceramide sensitizes tumor cell to Fas-mediated apoptosis. SW620 cells were treated with C16 ceramide for 1 h at the indicated concentrations (0 - 3 µM ), and then cultured in the absence or presence of FasL for approximately 24 h. |
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Reaction Conditions |
0 - 3 µM; 24h |
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Applications |
Exogenous C16 ceramide directly induced apoptosis in a dose-dependent manner, albeit at a low level, exogenous C16 ceramide significantly increased SW620 cell sensitivity to FasL-induced apoptosis。 |
| Animal experiment [2]: | |
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Animal models |
Male C57BL/6J mice |
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Preparation Method |
Mice received repeated DH (the dorsal hippocampus) or BLA (the basolateral amygdala) infusions of vehicle, C8 ceramide ((d18:1/8:0); 2 µM/0.2 µL/side), C16 ceramide ((d18:1/16:0); 2 µM/0.2 µL/side), or C20 ceramide ((d18:1/20:0); 2 µM/0.2 µL/side). |
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Dosage form |
2 µM/0.2 µL/side; Intracerebral infusions. |
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Applications |
C16 ceramide induced a depressive-like phenotype when infused into the DH(the dorsal hippocampus), but a predominantly non-social anxiogenic-like phenotype when infused into the BLA(the basolateral amygdala). |
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References: [1] Paschall AV, et al. Ceramide targets xIAP and cIAP1 to sensitize metastatic colon and breast cancer cells to apoptosis induction to suppress tumor progression. BMC Cancer. 2014 Jan 15;14:24. |
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| Cas No. | 24696-26-2 | SDF | |
| Synonyms | Palmitoyl Ceramide | ||
| Chemical Name | N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-hexadecanamide | ||
| Canonical SMILES | CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCCCCCCCCCCC)=O)CO | ||
| Formula | C34H67NO3 | M.Wt | 537.9 |
| Solubility | DMF : 10 mg/mL (ultrasonic and warming and heat to 55°C);Ethanol：3.3mg/mL | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8591 mL | 9.2954 mL | 18.5908 mL |
| 5 mM | 371.8 μL | 1.8591 mL | 3.7182 mL |
| 10 mM | 185.9 μL | 929.5 μL | 1.8591 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)