CellTracker Blue CMAC |
رقم الكتالوجGC20191
One-Click Copy Product Info
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CellTracker Blue CMAC is a live cell tracking probe (Ex/Em: 353/466nm) that emits bright blue fluorescence.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 147963-22-2
Sample solution is provided at 25 µL, 10mM.
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CellTracker Blue CMAC is a live cell tracking probe (Ex/Em: 353/466nm) that emits bright blue fluorescence[1]. CellTracker Blue CMAC can freely penetrate the cell membrane into the cell and converted into reaction products that are not cell membrane permeable. It can be used to monitor cell movement, localization, proliferation, migration, chemotaxis, and invasion.
References:
[1] Lee DY, Lim KS, Valencia GM, et al. One-Step Method for Instant Generation of Advanced Allogeneic NK Cells. Advanced Science. 2018 Nov;5(11):1800447.
This protocol only provides guidance; please modify it according to your specific needs.
1. Preparation of Staining Solution
(1) Prepare the stock solution: Dissolve CellTracker Blue CMAC powder in DMSO to prepare a stock solution with a concentration of 1-25mM.
Note: Unused stock solution should be aliquoted and stored at -20°C or -80°C in the dark to avoid repeated freeze-thaw cycles.
(2) Prepare the working solution: Dilute the stock solution with an appropriate buffer (e.g., serum-free culture medium or PBS) to prepare a working solution with a concentration of 0.5-25μM.
Note: Adjust the concentration of the working solution according to actual conditions, and prepare it fresh as needed.
2. Cell Suspension Staining
(1) Suspend cells: Centrifuge at 4°C, 1000g for 3-5min, discard the supernatant, and wash twice with PBS.
(2) For adherent cells: Wash twice with PBS, add trypsin to digest the cells, and after digestion is complete, centrifuge at 1000g for 3-5min.
(3) Add 1mL of CellTracker Blue CMAC working solution to resuspend the cells and incubate at 37°C in the dark for 15-45min. The optimal incubation time may vary for different cells; please adjust according to specific experimental requirements.
(4) After incubation, centrifuge at 1000g for 5min, remove the supernatant, and wash with PBS 2-3 times for 5min each.
(5) Resuspend the cells in pre-warmed serum-free cell culture medium or PBS.
3. Cell Adhesion Staining
(1) Culture adherent cells on sterile cover slips.
(2) Remove the cover slip from the culture medium, blot out excess medium, and place the cover slip in a humid environment.
(3) Add 100μL of the dye working solution from one corner of the cover slip and gently rock to ensure even coverage of all cells with the dye.
(4) Incubate at 37°C in the dark for 15-45min. The optimal incubation time may vary for different cells; please adjust according to specific experimental requirements.
(5) After incubation, aspirate the dye working solution and wash the cover slip with pre-warmed culture medium 2-3 times.
4. Microscope Detection: The maximum excitation/emission wavelengths for CellTracker Blue CMAC are 353/466nm.
Precautions:
①Fluorescent dyes are subject to quenching; please avoid light exposure as much as possible to slow down fluorescence quenching.
②For your safety and health, please wear a lab coat and disposable gloves when handling.
| Cas No. | 147963-22-2 | SDF | |
| Formula | C10H8ClNO2 | M.Wt | 209.63 |
| الذوبان | DMSO : 125 mg/mL (596.29 mM; Need ultrasonic) | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7703 mL | 23.8515 mL | 47.7031 mL |
| 5 mM | 954.1 μL | 4.7703 mL | 9.5406 mL |
| 10 mM | 477 μL | 2.3852 mL | 4.7703 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)