Cy5 carboxylic acid (non-sulfonated) |
رقم الكتالوجGC12471 |
Non-reactive fluorophore, for control experiments, and for calibration
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In the cells, The Cy5 carboxylic acid label on the dye can track the efficiency of the protein expression [1].
Reference:
[1] Deyle, K. M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry,2015, 7, 455–462.
Average Rating: 5
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