Dodecylphosphocholine-d38 (Synonyms: n-Dodecylphosphocholine-d38) |
رقم الكتالوجGC47259
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Dodecylphosphocholine-d38 هو الديوتيريوم المسمى Dodecylphosphocholine
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 130890-78-7
Sample solution is provided at 25 µL, 10mM.
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Dodecylphosphocholine-d38 is the deuterium-labeled form of dodecylphosphocholine, in which 38 hydrogen atoms are replaced by deuterium (²H)[1]. Dodecylphosphocholine-d38 is intended for use as an internal standard for the quantification of dodecylphosphocholine by GC- or LC-MS[2]. Dodecylphosphocholine-d38 is commonly used in nuclear magnetic resonance (NMR) studies of membrane protein structures[3].
References:
[1] Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.
[2] Kurauskas, V., Hessel, A., Ma, P., et al.How detergent impacts membrane proteins: Atomic-level views of mitochondrial carriers in dodecylphosphocholineJ. Phys. Chem. Lett.9(5)933-938(2018).
[3] Hwang PM, Choy WY, Lo EI, et al. Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci U S A. 2002;99(21):13560-13565.
PagP NMR Sample Preparation[1]
This plan is based on literature, please modify it to meet your specific needs.
1. Precipitated PagP was dissolved in 5mL of 6M Gdn-HCl, 50mM sodium phosphate (Ph=6.0). The final concentration was about 0.1mM.
2. The denatured solution was diluted dropwise into 35mL of 50mM sodium phosphate solution (pH=6.0), containing a final concentration of 0.2% Dodecylphosphocholine-d38.
3. The sample was dialyzed twice in 4L of 50mM sodium phosphate (pH=6.0) for 2h and then concentrated down to 0.45mL.
4. Finally, 50μL of D2O was added, yielding a 0.8mM PagP, 16% DPC NMR sample.
References:
[1] Hwang PM, Choy WY, Lo EI, et al. Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci U S A. 2002;99(21):13560-13565.
| Cas No. | 130890-78-7 | SDF | |
| المرادفات | n-Dodecylphosphocholine-d38 | ||
| Chemical Name | 2-[[(dodecyl-d25-oxy)hydroxyphosphinyl]oxy]-N,N,N-tri(methyl-d3)-ethan-1,1,2,2-d4-aminium, inner salt | ||
| Canonical SMILES | O=P(OC([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])(C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])[2H])[2H])([O-])OC([2H])([2H])C([2H])([2H])[N+](C([2H])([2H])[2H])(C([2H])([2H])[2H])C([2H])([2H])[2H] | ||
| Formula | C17D38NO4P | M.Wt | 389.7 |
| الذوبان | DMF: 16 mg/mL,DMSO: 16 mg/mL,Ethanol: 15 mg/mL,PBS (pH 7.2): 25 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5661 mL | 12.8304 mL | 25.6608 mL |
| 5 mM | 513.2 μL | 2.5661 mL | 5.1322 mL |
| 10 mM | 256.6 μL | 1.283 mL | 2.5661 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)