Ascr#2 (Synonyms: Ascaroside C6) |
رقم الكتالوجGC35402
One-Click Copy Product Info
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Ascr # 2 هو أسكاروزيد معزول عن Caenorhabditis elegans ، ويعزز بشكل فعال تشكيل dauer ، ويعمل أيضًا كجاذب ذكر قوي مع ascr # 3 بتركيز منخفض
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 946524-24-9
Sample solution is provided at 25 µL, 10mM.
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Ascr#2 is a type of ascaroside in Caenorhabditis elegans that can effectively induce the worm to enter the dauer stage (a dormant larval stage) with EC50 values of approximately 370nM at 25℃ and 1100nM at 20℃[1]. Ascr#2 exhibits a male-attracting effect on C. elegans at lower concentrations than those required for dauer induction when combined with Ascr#3[2]. Ascr#2-mediated effects are facilitated through G protein-coupled receptors (GPCRs) including DAF-37, DAF-38, SRBC-64 and SRBC-66[3][4].
In vitro, Ascr#2 (50, 100nM) treated HEK293 cells transfected with cMyc-DAF-37 and/or HA-DAF-38 for 30min enhanced the formation of DAF-37 homodimers as well as the heterodimers between DAF-37 and DAF-38[3]. Ascr#2 (1, 10μM) treated HEK293 cells coexpressing SRBC-64 and SRBC-66, but not each receptor alone, for 15min significantly inhibited forskolin-mediated increases in cAMP levels, which indicated that co-expression of SRBC-64 and -66 is sufficient to confer Ascr#2-dependent responses in vitro[4].
In vivo, Ascr#2 (100nM; 3days) promoted C. elegans development arrest and reduced developmental acceleration by nacq#1. The results confirmed the dauer-inducing effect of Ascr#2 and suggested the antagonism between nacq#1 and Ascr#2[5]. Ascr#2 (0.04, 4, and 400nM) treatment of C. elegans for up to 25 days enhanced both lifespan and stress resistance in a concentration-dependent manner. Ascr#2 (400nM) exposure on sir-2.1(ok434) mutant C. elegans for 25 days displayed lifespan regulation mediated by the sirtuin-dependent signaling pathway[6].
References:
[1] Butcher R.A., Fujita M., Schroeder F.C.,Jon Clardy J. Small-molecule pheromones that control dauer development in Caenorhabditis elegans.Nat Chem Biol. 2007 Jul;3(7):420-2.
[2] Choe, A.; von Reuss, S.H.; Kogan, D.; et al. Ascaroside Signaling is Widely Conserved Among Nematodes. Curr. Biol. 2012, 22, 772–780.
[3] Park D., O’Doherty I., Somvanshi R.K., et al. Interaction of structure-specific and promiscuous G-protein-coupled receptors mediates small-molecule signaling in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2012, 109, 9917–9922.
[4] KimK.H., Sato KJ, Shibuya M, et al. Two chemoreceptors mediate developmental effects of dauer pheromone in C. elegans. Science. 2009 Nov 13;326(5955):994-8.
[5] Ludewig A.H., Artyukhin B.A., Aprison E.Z., et al. An excreted small molecule promotes C. elegans reproductive development and aging. Nat Chem Biol. 2019 Aug;15(8):838-845.
[6] Ludewig A.H., Izrayelit Y., Park D., et al. Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1.Proc Natl Acad Sci USA. 2013 Mar 18;110(14):5522–5527.
| Cell experiment [1]: | |
Cell lines | HEK293 cells |
Preparation Method | Cotransfected HEK293 cells [cMyc-DAF37/HA-DAF-38, cMyc-DAF-37/HA-Rhodopsin, or cMyc-DAF37/SST5CR1] were treated with 50nM or 100nM Ascr#2 and/or 1μM somatostatin (SST) for 30min at 37 °C. |
Reaction Conditions | 50, 100nM; 30min |
Applications | Ascr#2 treatment enhanced the formation of DAF-37 homodimers as well as the heterodimers between DAF-37 and DAF-38. |
| Animal experiment [2]: | |
Animal models | wild-type (N2) C. elegans |
Preparation Method | Six-centimeter NGM plates containing Ascr#2, Ascr#3, 1:1 mixtures of Ascr#2 and Ascr#3, and mock-treated control plates were used. Late L4-stage worms were picked from synchronized NGM plates and transferred (25–30 worms per plate) to experimental plates at 20 °C. Survival was monitored by scoring for touch-provoked movement every other day, in some cases every day. |
Dosage form | 0.04, 4, 400nM; NGM plate; 25days |
Applications | Ascr#2 significantly increased mean lifespan of L4-stage C. elegans in a concentration-dependent manner. |
References: | |
| Cas No. | 946524-24-9 | SDF | |
| المرادفات | Ascaroside C6 | ||
| Canonical SMILES | CC(CC[C@H](O[C@H](O[C@@H](C)[C@@H](C1)O)[C@@H]1O)C)=O | ||
| Formula | C12H22O5 | M.Wt | 246.3 |
| الذوبان | DMSO : 400 mg/mL (1624.04 mM; Need ultrasonic) | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0601 mL | 20.3004 mL | 40.6009 mL |
| 5 mM | 812 μL | 4.0601 mL | 8.1202 mL |
| 10 mM | 406 μL | 2.03 mL | 4.0601 mL |
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- Purity: >98.00% Appearance: A liquid
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)