PC DBCO-PEG4-NHS ester (Synonyms: PC DBCO-PEG4-NHS ester) |
رقم الكتالوجGF04904
One-Click Copy Product Info
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PC DBCO-PEG4-NHS ester is a PEG based PROTAC linker that can be used as a linker in click chemistry for the synthesis of PROTAC molecules.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2055025-02-8
Sample solution is provided at 25 µL, 10mM.
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PC DBCO-PEG4-NHS ester is a PEG based PROTAC linker that can be used as a linker in click chemistry for the synthesis of PROTAC molecules. The reaction between the DBCO group in PC DBCO-PEG4-NHS ester and azide compounds (usually azide methane (CH2N2) or azide amide analogues) belongs to the stress promoted azide alkyne cycloaddition reaction (SPAAC), which is a metal free chemical reaction. The alkyne portion of DBCO can bind with azide groups and form a stable 1,2,3-triazole cyclobutane compound through a [3+2] cycloaddition mechanism.
PC DBCO-PEG4-NHS ester can be used for surface modification of samples such as peptides, proteins, enzymes, and live cells, to prepare protein peptide conjugates, protein/peptide oligonucleotide conjugates, and antibody enzyme/antibody drug conjugates. PC DBCO-PEG4-NHS ester only reacts with azide compounds, exhibiting good bioorthogonality, high selectivity, and high reaction rate. Due to the absence of metal catalysts in the reaction, this product is also suitable for use in living organisms or other metal sensitive environments, and has broad application prospects and scientific value.
It is recommended to explore the labeling ratio of PC DBCO-PEG4-NHS ester to ligand at around 5:1 or 8:1, and label at room temperature for 30-1h. The usage conditions need to be adjusted according to specific experimental needs and labeling effects.
| Cas No. | 2055025-02-8 | SDF | |
| المرادفات | PC DBCO-PEG4-NHS ester | ||
| Formula | C47H55N5O16 | M.Wt | 945.96 |
| الذوبان | DMSO : 250 mg/mL (264.28 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0571 mL | 5.2856 mL | 10.5713 mL |
| 5 mM | 211.4 μL | 1.0571 mL | 2.1143 mL |
| 10 mM | 105.7 μL | 528.6 μL | 1.0571 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)