MTS Reagent, cell proliferation assay reagent |
رقم الكتالوجGF07333
One-Click Copy Product Info
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MTS Reagent, cell proliferation assay reagent is a novel water-soluble tetrazolium salt compound.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 138169-43-4
Sample solution is provided at 25 µL, 10mM.
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MTS Reagent, cell proliferation assay reagent is a novel water-soluble tetrazolium salt compound. MTS Reagent can be reduced by dehydrogenases in the mitochondria of living cells to generate a water-soluble formazan dye. The absorbance of this product at 490nm is proportional to the number and metabolic activity of viable cells in the culture system. MTS Reagent can be used for rapid and highly sensitive detection of cell proliferation, cytotoxicity, and chemical sensitivity[1-4].
References:
[1] Kabakov AE, Gabai VL. Cell Death and Survival Assays. Methods Mol Biol. 2018;1709:107-127.
[2] Zheng X, Chang F, Rong Y, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker, promotes proliferation and invasion of triple negative breast cancer. Exp Mol Pathol. 2022 Apr;125:104751.
[3] Soto-Gamez A, Wang Y, Zhou X, et al. Enhanced extrinsic apoptosis of therapy-induced senescent cancer cells using a death receptor 5 (DR5) selective agonist. Cancer Lett. 2022 Jan 28;525:67-75.
[4] Singh RR, Kulshreshtha R, Popat A. Ultrasmall nanoparticles for co-delivery of antisense oligonucleotides targeting miR-21 and miR-210 to treat glioblastoma. J Nanobiotechnology. 2025 Jul 2;23(1):482.
MTS Reagen for Detecting Cell Viability
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Preparation of MTS Working Solution
(1) Preparation of Stock Solution: Weigh 42mg of MTS reagent powder and add to 21mL of DPBS. Mix on a magnetic stirrer at moderate speed for 15 minutes or until the MTS Reagen is completely dissolved. Measure the pH of the MTS solution. The optimal pH range is 6.0–6.5. If the pH exceeds 6.5, adjust to pH=6.5 using 1N HCl. Dissolve 38.3mg of phenazine methosulfate (PMS) in 10mL of DPBS. Sterilize the MTS Reagen solution by filtering through a 0.2μm membrane. Store the solution in the dark at -20°C.
(2) Preparation of Working Solution: Prepare 2.1mL of working solution by combining 2mL of MTS Reagen stock solution with 100μL of PMS solution. Mix gently by swirling or pipetting.
2. Cell Treatment and Staining
(1) Cell Seeding: Seed cells into a 96-well plate, typically adding 100μL of culture medium per well.
(2) Staining: Add 20μL of the MTS Reagen/PMS working solution to each well of the 96-well plate (containing 100μL of medium per well). Gently mix the plate to ensure uniform distribution.
(3) Incubation: Incubate the plate at 37°C in a humidified 5% CO₂ atmosphere for 1–4 hours, protected from light.
3. Signal Detection
(1) Immediate Measurement: After incubation, immediately measure the absorbance at 490nm using a microplate reader.
(2) Delayed Measurement: To delay measurement, add 25μL of 10% SDS to each well to terminate the reaction. Store the plate protected from light in a humidified chamber at room temperature for up to 18 hours before measurement. Note: The optimal detection wavelength is 490 nm, but measurements can be taken within 450–540nm. A reference wavelength of 630–700nm may be used to eliminate background interference.
Precautions:
(1) MTS reagent is light-sensitive. Perform all steps protected from light.
(2) Strong reducing agents (e.g., vitamin C, thiol-containing compounds) or high pH in the culture medium may increase background absorbance. Control experimental conditions carefully.
(3) PMS is a carcinogen. Dispose of waste according to safety regulations.
(4) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Martin F, Neubert A, Lutter AH, et al. MTS, WST-8, and ATP viability assays in 2D and 3D cultures: Comparison of methodologically different assays in primary human chondrocytes. Clin Hemorheol Microcirc. 2024;88(s1):S3-S19.
| Cas No. | 138169-43-4 | SDF | |
| Formula | C20H17N5O6S2 | M.Wt | 487.5089 |
| الذوبان | Storage | -20°C,away from moisture, sealed storage,protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0512 mL | 10.2562 mL | 20.5124 mL |
| 5 mM | 410.2 μL | 2.0512 mL | 4.1025 mL |
| 10 mM | 205.1 μL | 1.0256 mL | 2.0512 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)