Glycogen |
رقم الكتالوجGC33804
One-Click Copy Product Info
|
الجليكوجين عبارة عن وسيط حال للجلوكوز وفوسفات عالي الطاقة يمكن أن يكون بمثابة شكل من أشكال تخزين الطاقة في البشر والحيوانات والفطريات والبكتيريا.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9005-79-2
Sample solution is provided at 25 µL, 10mM.
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Glycogen is a polysaccharide which serves as a form of energy storage in animals and fungi [1]. It is mainly found in the liver and muscle tissues as a secondary long-term energy storage molecule[2]. Glycogen is well-studied in research on cellular metabolism[3], diabetes[4], and athletic performance[5]. In addition, Glycogen is also used as a carrier to help the precipitation and recovery of small amounts of DNA and RNA during nucleic acid extraction, improving the yield and purity of the extracted nucleic acids[6][7].
References:
[1]. Roach PJ, Depaoli-Roach AA, Hurley TD, et al. Glycogen and its metabolism: some new developments and old themes. Biochem J. 2012 Feb 1;441(3):763-87. doi: 10.1042/BJ20111416. PMID: 22248338; PMCID: PMC4945249.
[2]. Kanungo S, Wells K, Tribett T, et al. Glycogen metabolism and glycogen storage disorders. Ann Transl Med. 2018 Dec;6(24):474. doi: 10.21037/atm.2018.10.59. PMID: 30740405; PMCID: PMC6331362.
[3]. Adeva-Andany MM, González-Lucán M, Donapetry-García C, et al. Glycogen metabolism in humans. BBA Clin. 2016 Feb 27;5:85-100. doi: 10.1016/j.bbacli.2016.02.001. PMID: 27051594; PMCID: PMC4802397.
[4]. Barroso E, Jurado-Aguilar J, Wahli W, et al. Increased hepatic gluconeogenesis and type 2 diabetes mellitus. Trends Endocrinol Metab. 2024 May 29:S1043-2760(24)00124-3. doi: 10.1016/j.tem.2024.05.006. Epub ahead of print. PMID: 38816269.
[5]. Vigh-Larsen JF, Ørtenblad N, Spriet LL, et al. Muscle Glycogen Metabolism and High-Intensity Exercise Performance: A Narrative Review. Sports Med. 2021 Sep;51(9):1855-1874. doi: 10.1007/s40279-021-01475-0. Epub 2021 Apr 26. PMID: 33900579.
[6]. Shaw KJ, Thain L, Docker PT, et al. The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Anal Chim Acta. 2009 Oct 12;652(1-2):231-3. doi: 10.1016/j.aca.2009.03.038. Epub 2009 Mar 31. PMID: 19786185.
[7]. Razak MR, Aris AZ, Md Yusoff F, et al. Assessment of RNA extraction protocols from cladocerans. PLoS One. 2022 Apr 26;17(4):e0264989. doi: 10.1371/journal.pone.0264989. PMID: 35472091; PMCID: PMC9041806.
Experimental Protocol for Glycogen in Nucleic Acid Precipitation Assay
1. Dissolve glycogen in nuclease-free sterile water. After complete dissolution, filter through a 0.22µm sterile, enzyme-free filter. Prepare a stock solution with a concentration of 20µg/µL. Aliquot and store at -20°C or -80°C.
2. Estimate the volume of the nucleic acid solution. If the solution contains a high concentration of salts, dilute with TE (pH 7.0) and mix well. If the solution has a low salt concentration, add 0.1 volume of 3M sodium acetate.
3. Add Glycogen to a final concentration of 0.05-1µg/µL to facilitate precipitation and maximize recovery.
4. Add 2.5–3.0 volumes of ice-cold ethanol (or 1 volume of isopropanol) and mix the solution well. Store the ethanolic solution for 1h to overnight at −20°C to allow nucleic acid to precipitate. Centrifuge the mixture at 12,000-15,000g for 10 minutes at 4°C. Carefully remove the supernatant without disturbing the nucleic acid pellet.
5. Wash the pellet with 0.5-1mL of cold 70% ethanol. Vortex briefly and centrifuge again at 12,000-15,000g for 5 minutes at 4°C. Remove the supernatant and air-dry the pellet for 5-10 minutes to remove any residual ethanol.
6. Resuspend the nucleic acid pellet in an appropriate volume of nuclease-free water or TE buffer. Mix gently to ensure complete dissolution of the nucleic acid.
This protocol only provides a guideline and should be modified according to your specific needs.
References:
[1]. Green MR, Sambrook J. Precipitation of RNA with Ethanol. Cold Spring Harb Protoc. 2020 Mar 2;2020(3):101717. doi: 10.1101/pdb.prot101717. PMID: 32123016.
| Cas No. | 9005-79-2 | SDF | |
| Canonical SMILES | [Glycogen] | ||
| Formula | C24H42O21 | M.Wt | 666.57 |
| الذوبان | Water : 50 mg/mL (75.01 mM) | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5002 mL | 7.5011 mL | 15.0022 mL |
| 5 mM | 300 μL | 1.5002 mL | 3.0004 mL |
| 10 mM | 150 μL | 750.1 μL | 1.5002 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)