Pyroxamide |
رقم الكتالوجGC11095
One-Click Copy Product Info
|
A HDAC inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 382180-17-8
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Pyroxamide is an inhibitor of histone deacetylase [1].
Histone deacetylases (HADC) are a series of enzymes that remove acetyl groups from an ε-N-acetyl lysine amino acid on a histone and make the histones to wrap the DNA more tightly, which prevent transcription.
In murine erythroleukemia (MEL) cells, pyroxamide induced terminal differentiation and inhibited cells growth by apoptosis or cell cycle arrest. Also, pyroxamide increased the levels of acetylated histones H2A, H2B, H3 and H4. Pyroxamide inhibited HDAC1 activity with ID50 value of 100 nM [1]. In RD (embryonal) and RH30B cell lines, pyroxamide (1.25-20.0 μM) induced apoptosis and accumulation of acetylated histones. Also, pyroxamide induced the expression of p21/WAF1 protein and increased the sub-G1 fraction [2].
In mice bearing human CWR22 prostate cancer xenograft, pyroxamide (100 or 200 mg/kg) significantly suppressed the growth of the tumor and increased the expression of p21/WAF1 protein in a dose-dependent way [1].
References:
[1]. Butler LM, Webb Y, Agus DB, et al. Inhibition of transformed cell growth and induction of cellular differentiation by pyroxamide, an inhibitor of histone deacetylase. Clin Cancer Res, 2001, 7(4): 962-970.
[2]. Kutko MC, Glick RD, Butler LM, et al. Histone deacetylase inhibitors induce growth suppression and cell death in human rhabdomyosarcoma in vitro. Clin Cancer Res, 2003, 9(15): 5749-5755.
| Cas No. | 382180-17-8 | SDF | |
| Chemical Name | N'-hydroxy-N-pyridin-3-yloctanediamide | ||
| Canonical SMILES | C1=CC(=CN=C1)NC(=O)CCCCCCC(=O)NO | ||
| Formula | C13H19N3O3 | M.Wt | 265.31 |
| الذوبان | DMF: 2 mg/ml,DMSO: 5 mg/ml,DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7692 mL | 18.8459 mL | 37.6918 mL |
| 5 mM | 753.8 μL | 3.7692 mL | 7.5384 mL |
| 10 mM | 376.9 μL | 1.8846 mL | 3.7692 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)