ZINC69391 |
رقم الكتالوجGC66020
One-Click Copy Product Info
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يتداخل ZINC69391 ، وهو مثبط محدد لـ Rac1 ، مع تفاعل Rac1-GEF عن طريق إخفاء بقايا Trp56 على سطح Rac1. يتداخل ZINC69391 مع تفاعل Rac1 مع Dock180 ويقلل مستويات Rac1-GTP. يستحث ZINC69391 موت الخلايا المبرمج ، ويظهر تأثيرات مضادة للتكاثر ومضاد للقسطرة.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 303094-67-9
Sample solution is provided at 25 µL, 10mM.
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ZINC69391, a specific Rac1 inhibitor, interferes with Rac1-GEF interaction by masking Trp56 residue on Rac1 surface. ZINC69391 interferes with the interaction of Rac1 with Dock180 and reduces Rac1-GTP levels. ZINC69391 induces apoptosis, and shows antiproliferative and antimetastatic effects[1][2][3].
ZINC69391 inhibits growth of U937, HL-60, KG1A and Jurkat cells with IC50s ranging from 41 to 54 μM[1].
ZINC69391 (50-100 μM; 24 hours) augments the enzymatic activity of caspase 3 in a concentration dependent manner[1].
ZINC69391 (0-125 μM; 72h) reduces cell proliferation of human glioma cells[2].
ZINC69391 (50-100 μM; 48 hours) triggers cell cycle arrest[2].
ZINC69391 (50 μM; 24 hours) triggers apoptosis on human acute leukemic cells[1].
Cell Proliferation Assay[2]
| Cell Line: | U-87 MG, LN229 cells |
| Concentration: | 0-125μM |
| Incubation Time: | 72 hours |
| Result: | Reduced cell proliferation in a concentration-dependent manner. |
Cell Cycle Analysis[2]
| Cell Line: | LN229 cells |
| Concentration: | 50, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Resulted in a significant increased percentage of cells in the sub-G0/G1 phase in a concentration dependent manner. |
Apoptosis Analysis[1]
| Cell Line: | HL-60, U937 and KG1A cell lines |
| Concentration: | 50 μM |
| Incubation Time: | 24 hours |
| Result: | Led to a significant increase in apoptotic cells. |
ZINC69391 (25 mg/kg; i.p; daily for 21 days) impairs metastatic lung colonization in a syngeneic animal model[3].
| Animal Model: | Specific pathogen-free female BALB/c inbred mice (bearing F3II cells)[3] |
| Dosage: | 25 mg/kg body weight |
| Administration: | I.p; daily for 21 days |
| Result: | Significantly reduced by about 60% the formation of total metastatic lung colonies. |
| Cas No. | 303094-67-9 | SDF | |
| Formula | C14H14F3N5 | M.Wt | 309.29 |
| الذوبان | DMSO : 25 mg/mL (80.83 mM; ultrasonic and warming and heat to 60°C) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2332 mL | 16.1661 mL | 32.3321 mL |
| 5 mM | 646.6 μL | 3.2332 mL | 6.4664 mL |
| 10 mM | 323.3 μL | 1.6166 mL | 3.2332 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)