Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt) |
| Catalog No.GC48995 |
A fluorogenic substrate of enteropeptidase
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage.1,2 Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 380/500 nm.3
1.Pepeliaev, S., Krahulec, J., ?ernÝ, Z., et al.High level expression of human enteropeptidase light chain in Pichia pastorisJ. Biotechnol.156(1)67-75(2011) 2.Choi, M.-G., Lee, S., Chung, H.-S., et al.A fluorogenic method for measuring enteropeptidase activity: Spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarinBMB Rep.44(7)458-461(2011) 3.Gossrau, R.Cytochemistry of membrane proteasesHistochem J.17(7)737-771(1985)
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