Flagelin 22 (Flagellin 22) (Synonyms: Flagellin 22) |
Catalog No.GC32202
One-Click Copy Product Info
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Flagelin 22 (Flagellin 22), un fragmento de la flagelina bacteriana, es un eficaz inductor tanto en plantas como en algas.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 304642-91-9
Sample solution is provided at 25 µL, 10mM.
Flagelin 22 (Flagellin 22), un fragmento de la flagelina bacteriana, es un eficaz inductor tanto en plantas como en algas[1].
Flagelina 22 es un péptido de 22 aminoácidos que corresponde a la región N-terminal altamente conservada de la flagelina, capaz de inducir reacciones de inmunidad en diversas plantas como el tomate, la papa, el tabaco y Arabidopsis thaliana. Además, Flagelina 22 inhibe significativamente el crecimiento de algas a una concentración de 1 μM[1]. El tratamiento de células de tabaco con 200 nM de Flagelina 22 indujo la producción de 13 metabolitos. En comparación con otras moléculas de patrones moleculares asociados a microbios (MAMP) y hormonas vegetales, las células tratadas con Flagelina 22 produjeron un metabolito único, el isómero 3 de feruloiltirosina glucósido [2]. Flagelina 22 (100 nM) activa la inmunidad inducida por patrones (PTI) en plantas, lo que conduce a una disminución en la expresión de genes involucrados en la biosíntesis de la hormona promotora del crecimiento brasinoesteroide (BR)[3].
References:
[1] Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801.
[2] Mhlongo M I, Piater L A, Madala N E, et al. Phenylpropanoid defences in Nicotiana tabacum cells: Overlapping metabolomes indicate common aspects to priming responses induced by lipopolysaccharides, chitosan and flagellin-22[J]. PLoS One, 2016, 11(3): e0151350.
[3] Jiménez-Góngora T, Kim SK, Lozano-Durán R, Zipfel C. Flg22-Triggered Immunity Negatively Regulates Key BR Biosynthetic Genes. Front Plant Sci. 2015 Nov 9;6:981.
| Experimentos celulares [1]: | |
Líneas celulares | Células de tabaco |
Método de preparación | Las células de tabaco fueron inducidas con MAMPs (como lipopolisacáridos, quitina y Flagelina 22) y fitohormonas (ácido abscísico, ácido metiljasmonico y ácido metiljasmonico). Las células se cosecharon en diferentes momentos para extraer metabolitos. |
Condiciones de reacción | 200 nM, 0, 6, 12, 18 y 24 h |
Áreas de aplicación | Las células tratadas con Flagelina 22 indujeron la producción de 13 metabolitos, y en comparación con otros inductores, las células tratadas con Flagelina 22 presentaron un metabolito único, el isómero 3 de feruloiltirosina glucósido. |
Referencias: [1] Mhlongo M I, Piater L A, Madala N E, et al. Defensas fenilpropanoides en células de Nicotiana tabacum: los metabolomas superpuestos indican aspectos comunes en las respuestas de precondicionamiento inducidas por lipopolisacáridos, quitina y flagelina-22[J]. PLoS One, 2016, 11(3): e0151350. | |
| Cas No. | 304642-91-9 | SDF | |
| Sinónimos | Flagellin 22 | ||
| Canonical SMILES | Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala | ||
| Formula | C93H162N32O34 | M.Wt | 2272.48 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 440 μL | 2.2002 mL | 4.4005 mL |
| 5 mM | 88 μL | 440 μL | 880.1 μL |
| 10 mM | 44 μL | 220 μL | 440 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)