H-D-Pro-Phe-Arg-pNA . 2 HCl (Synonyms: pFR-pNA, Plasma Kallikrein Chromogenic Substrate, D-Pro-Phe-Arg-p-nitroaniline) |
Catalog No.GA22303
One-Click Copy Product Info
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H-D-Pro-Phe-Arg-pNA . 2 HCl is a chromogenic peptide substrate that has been used for the determination of kallikreins.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62354-56-7
Sample solution is provided at 25 µL, 10mM.
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H-D-Pro-Phe-Arg-pNA . 2 HCl is a chromogenic peptide substrate that has been used for the determination of kallikreins [1]. H-D-Pro-Phe-Arg-pNA . 2 HCl can be cleaved by Kallikreins, releasing p-nitroaniline (pNA), and the release rate is determined by spectrophotometry at 405nm[2]. H-D-Pro-Phe-Arg-pNA . 2 HCl can serve as a substrate for bovine trypsin and be used for screening irreversible serine protease inhibitors[3].
References:
[1] Shibuya Y, Yamamoto T, Tanaka H, et al. Mechanized assay of plasma prekallikrein by activation with Pseudomonas aeruginosa elastase and amidolysis of chromogenic substrate[J]. Clinica chimica acta, 1991, 200(2-3): 119-127.
[2] Shin I S, Shim Y B, Hong C M, et al. An improved, reliable and practical kinetic assay for the detection of prekallikrein activator in blood products[J]. Archives of pharmacal research, 2002, 25(4): 505-510.
[3] Zhao Y, Jin Y, Wei S S, et al. Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi[J]. Toxicon, 2005, 46(6): 635-640.
| Cas No. | 62354-56-7 | SDF | |
| Sinónimos | pFR-pNA, Plasma Kallikrein Chromogenic Substrate, D-Pro-Phe-Arg-p-nitroaniline | ||
| Formula | C26H34N8O5 · 2 HCl | M.Wt | 611.53 |
| Solubility | DMF: 10 mg/ml,PBS (pH 7.2): 5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6352 mL | 8.1762 mL | 16.3524 mL |
| 5 mM | 327 μL | 1.6352 mL | 3.2705 mL |
| 10 mM | 163.5 μL | 817.6 μL | 1.6352 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)