LAH4 TFA |
Catalog No.GC62281
One-Click Copy Product Info
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LAH4 TFA, una hélice alfa del antibiÓtico peptÍdico anfipÁtico diseÑado, exhibe potentes actividades antimicrobianas, de transfecciÓn de Ácidos nucleicos y de penetraciÓn celular.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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LAH4 TFA, an alpha-helix of the designed amphipathic peptide antibiotic, exhibits potent antimicrobial, nucleic acid transfection and cell penetration activities. LAH4 TFA possesses high plasmid DNA delivery capacities. LAH4 TFA has a strong affinity for anionic lipids found in the outer membrane of bacterial membranes[1][2][3].
LAH4 TFA is capable of complexing DNA, associating with the cell surfacemembrane and then, when enveloped within an endosome, disrupts the endosomal membrane as the pH drops[1].LAH4 TFA possesses robust plasmid DNA transfection properties. Peptides of the LAH4 family are able to efficiently deliver siRNAs in vitro into a human cell line[2].LAH4 TFA is found to mediate the intracellular delivery of both protein and nucleotide cargo and facilitate protein internalization using mechanisms involving endosomal acidification and processing through the proteasome pathway, leading to enhanced cross presentation of protein antigen by dendritic cells to CD8+ T cells. LAH4 TFA also improves the internalization of CpG, resulting in NF-kB activation, thus potentiating the adjuvant effect of CpG[3]. LAH4 TFA exhibits antibiotic activities against Escherichia coli and Bacillus subtilis. the peptide does not, however, lyse human red blood cells at bacteriocidal concentrations. The antibiotic activities of LAH4 TFA are 2 orders of magnitude more pronounced at pH 5 when compared with pH 7.5[4].
[1]. Mason AJ, et al. The antibiotic and DNA-transfecting peptide LAH4 selectively associates with, and disorders, anionic lipids in mixed membranes. FASEB J. 2006;20(2):320-322.
[2]. Langlet-Bertin B, et al. Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery. Pharm Res. 2010;27(7):1426-1436.
[3]. Zhang TT, et al. LAH4 enhances CD8+ T cell immunity of protein/peptide-based vaccines. Vaccine. 2012;30(4):784-793.
| Cas No. | SDF | ||
| Formula | C134H229F3N38O29 | M.Wt | 2893.55 |
| Solubility | Water : 100 mg/mL (34.56 mM; Need ultrasonic) | Storage | -20°C, away from moisture |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 345.6 μL | 1.728 mL | 3.456 mL |
| 5 mM | 69.1 μL | 345.6 μL | 691.2 μL |
| 10 mM | 34.6 μL | 172.8 μL | 345.6 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)