PE-CF594

PE-CF594 is a labeled monoclonal antibody conjugate that specifically binds to HLA-DR on the surface of monocytes and B cells, while acting as a signal attenuator. Through steric hindrance and a possible fluorescence resonance energy transfer mechanism, PE-CF594 specifically reduces the fluorescence intensity of PE-CD124 staining, but does not interfere with the staining of other PE-labeled antibodies such as CD40, CD4 or CD14. PE-CF594 can also be used to detect the emission signal of mt-Keima after excitation with a 561-nm laser, thereby effectively evaluating mitophagy activity ^{[1] [2]}.

In Vitro, PE-CF594 enables specific, sensitive detection of mt-Keima fluorescence in HeLa-Parkin cells stably expressing mt-Keima, supporting quantitative flow cytometric analysis of mitophagy activity^[2]. PE-CF594 (0-25.0 μg/L; 3 min) can be quantified and discriminated from strongly overlapping fluorochromes using both compensated band pass filters and MOE-based optical computing, with MOE systems enabling multiplexed detection with two elements instead of three discrete filters^[3].

References:
[1]. De Vita M, et al. Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens. Cytometry B Clin Cytom. Published online October 1, 2014.
[2]. Um JH, et al. Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima. J Vis Exp. 2018;(138):58099. Published 2018 Aug 12.
[3]. Priore R J, et al. Multivariate optical computing for fluorochrome discrimination[C]//Optical Diagnostics and Sensing XV: Toward Point-of-Care Diagnostics. SPIE, 2015, 9332: 163-170.