Quadrol |
Catalog No.GC31784
One-Click Copy Product Info
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Quadrol es un inmunoestimulante y ha sido implicado como un agente potencialmente Útil en la cicatrizaciÓn acelerada de heridas.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 102-60-3
Sample solution is provided at 25 µL, 10mM.
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Quadrol is an immunostimulant and has been implicated as a potentially useful agent in accelerated wound healing.
Results show that viability of macrophages incubated with Quadrol at concentrations of 0.5 mM, 1 mM and 4mM are identical to that for controls. Viability, however, is reduced to 50% of control at concentrations of 16 mM to 32 mM. After four hours, at concentrations of 1 mM and 4 mM, Quadrol produces enhanced spreading of 88% and 80%, respectively, as compared to the control of 28%. Quadrol, at a concentration of 16 mM, shows reduced percentage of spreading of macrophages after four hours. Exposure of macrophages to 1.0 mM or 4.0 mM Quadrol concentrations enhances phagocytic activity, 41% and 57%, respectively, over that of the cells exposed to media alone (34%)[1].
On day eight after the implantation, the amount of collagen in the implants of normal mice injected with Quadrol exceeds controls by more than 200% (p<0.025). By day 11, the collagen content increases to over 300% higher than controls (p<0.01) and by the end of two weeks after wounding, the time interval typically required for normal and complete wound healing, the collagen accumulated in the implants of the Quadrol-treated mice is about 50% above control (p<0.1). The accumulation of collagen in the implants of Quadrol treated STZ-diabetic mice about 100% above the untreated diabetic control on days 8 to 11. By day 14, the collagen deposition has increased to 200% above the controls (p<0.0 5)[2].
[1]. Bhide MV, et al. In vitro stimulation of macrophages by quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine]. J Immunopharmacol. 1985;7(3):303-12. [2]. Bhide MV, et al. Promotion of wound collagen formation in normal and diabetic mice by quadrol. Immunopharmacol Immunotoxicol. 1988;10(4):513-22.
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Cell experiment: |
The standard trypan blue exclusion test is performed to measure cell viability (10). Cells are exposed to increasing concentrations of Quadrol (0.5 mM to 32 mM) and incubated at 37°C for varying lengths of time before treatment with trypan blue[1]. |
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Animal experiment: |
At the time of polytetrafluoroethylene (PTFE) tubing implantation, six test groups (3 streptozotocin-induced (STZ) diabetic and 3 non-diabetic) consisting of 5 to 7 animals per sampling time receive either diluent (control group) or Quadrol (10 mM) injected approximately 1 cm from the implantation site. The PTFE implants recovered from the mice after 2 to 14 days are stored frozen until processed and then analyzed by the HPLC method[2]. |
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References: [1]. Bhide MV, et al. In vitro stimulation of macrophages by quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine]. J Immunopharmacol. 1985;7(3):303-12. |
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| Cas No. | 102-60-3 | SDF | |
| Canonical SMILES | CC(O)CN(CC(O)C)CCN(CC(O)C)CC(O)C | ||
| Formula | C14H32N2O4 | M.Wt | 292.41 |
| Solubility | DMSO : ≥ 83.3 mg/mL (284.87 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4199 mL | 17.0993 mL | 34.1986 mL |
| 5 mM | 684 μL | 3.4199 mL | 6.8397 mL |
| 10 mM | 342 μL | 1.7099 mL | 3.4199 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A jelly
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)