24-dehydro Cholesterol-d6 (Synonyms: Desmosterol-d6) |
| رقم الكتالوجGC46243 |
An internal standard for the quantification of 24-dehydro cholesterol
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1246298-67-8
Sample solution is provided at 25 µL, 10mM.
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24-dehydro Cholesterol-d6 is intended for use as an internal standard for the quantification of 24-dehydro cholesterol by GC- or LC-MS. 24-dehydro Cholesterol is an immediate precursor to cholesterol in the Bloch pathway of cholesterol biosynthesis. Structurally, it varies from cholesterol only by a single double bond at carbon 24 and has been used as cholesterol substitute in cellular membrane studies.[1] During brain development, 24-dehydro cholesterol transiently accumulates, composing up to 30% of total brain sterol, where it is poised to rapidly enrich membrane sterols.[2] However, defects in cholesterol synthesis can lead to a condition called desmosterolosis, which results in an accumulation of excess 24-dehydro cholesterol.[3] 24-dehydro Cholesterol has been reported to activate liver X receptor-target genes in both the liver of cholesterol-free mice and in cholesterol-starved macrophage foam cells in atherosclerotic lesions.[4],[5]
Reference:
[1]. Huster, D., Scheidt, H.A., Arnold, K., et al. Desmosterol may replace cholesterol in lipid membranes. Biophys. J. 88(3), 1838-1844 (2005).
[2]. Jansen, M., Wang, W., Greco, D., et al. What dictates the accumulation of desmosterol in the developing brain? FASEB J. 27(3), 865-870 (2013).
[3]. Clayton, P.T. Disorders of cholesterol biosynthesis. Arch. Dis. Child. 78(2), 185-189 (1998).
[4]. Heverin, M., Meaney, S., Brafman, A., et al. Studies on the cholesterol-free mouse: Strong activation of LXR-regulated hepatic genes when replacing cholesterol with desmosterol. Arterioscler. Thromb. Vasc. Biol. 27(10), 2191-2197 (2007).
[5]. Spann, N.J., Garmire, L.X., McDonald, J.G., et al. Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses. Cell 151(1), 138-152 (2012).
| Cas No. | 1246298-67-8 | SDF | |
| المرادفات | Desmosterol-d6 | ||
| Chemical Name | cholesta-5,24-dien-26,26,26,27,27,27-d6-3β-ol | ||
| Canonical SMILES | C[C@H](CC/C=C(C([2H])([2H])[2H])/C([2H])([2H])[2H])[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| Formula | C27H38D6O | M.Wt | 390.7 |
| الذوبان | 0.25mg/mL in ethanol, 50mg/mL in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5595 mL | 12.7975 mL | 25.5951 mL |
| 5 mM | 0.5119 mL | 2.5595 mL | 5.119 mL |
| 10 mM | 0.256 mL | 1.2798 mL | 2.5595 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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