Anisomycin (Synonyms: Flagecidin, NSC 76712, Wuningmeisu C) |
رقم الكتالوجGC11559
One-Click Copy Product Info
|
محفز JNK، قوي ومحدد بشكل كبير
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 22862-76-6
Sample solution is provided at 25 µL, 10mM.
Anisomycin, an antibiotic isolated from Streptomyces griseus, is also a JNK activator. Anisomycin inhibited protein synthesis mainly by inhibiting ribosomal subunit peptidyl transferase activity at 80S. Additionally, anisomycin induces apoptosis in various cell types through the activation of the P38, JNK, and ERK1/2 pathways, making it a promising chemotherapeutic drug for cancer[1-4].
Anisomycin(10-100nM; 24h) reduces cell viability in a concentration-dependent manner in B16 melanoma cells[5]. Anisomycin(1-80 ng/ml; 24h) may activate the P53/P21/P27 signaling pathway to reduce the expression of ICBP90, inhibit the expression of P-CDK2 to block cells in the S and G2/M phases, and ultimately lead to the inhibition of Jurkat T cell proliferation [6]. Anisomycin(31.8 μM) inhibits the proliferation, migration, and angiogenesis of OCSCs by altering the expression of antisense RNA NCBP2-AS2[7].
Anisomycin(5 mg/kg; i.p; 3 weeks) effectively disrupts breast cancer angiogenesis by impairing capillary network formation, inhibiting cell migration, proliferation, and survival in MD-MBA-231 cell-based breast cancer models[8]. Anisomycin(2-200μg/kg; i.p; 30days) upregulates utrophin protein levels in mdx mouse diaphragm[9].
References：
[1]. Grollman AP. Inhibitors of protein biosynthesis. II. Mode of action of anisomycin. J Biol Chem. 1967 Jul 10;242(13):3226-33. PMID: 6027796.
[2]. Hall SS, Loebenberg D, et,al. Structure-activity relationships of synthetic antibiotic analogues of anisomycin. J Med Chem. 1983 Apr;26(4):469-75. doi: 10.1021/jm00358a003. PMID: 6834379.
[3]. Schmeits PC, Katika MR, et,al. DON shares a similar mode of action as the ribotoxic stress inducer anisomycin while TBTO shares ER stress patterns with the ER stress inducer thapsigargin based on comparative gene expression profiling in Jurkat T cells. Toxicol Lett. 2014 Jan 30;224(3):395-406. doi: 10.1016/j.toxlet.2013.11.005. Epub 2013 Nov 15. PMID: 24247028.
[4]. Slipicevic A, Øy GF, et,al. Low-dose anisomycin sensitizes melanoma cells to TRAIL induced apoptosis. Cancer Biol Ther. 2013 Feb;14(2):146-54. doi: 10.4161/cbt.22953. Epub 2012 Nov 28. PMID: 23192275; PMCID: PMC3571996.
[5]. Ushijima H, Monzaki R, et,al. Suppressive Effects of Anisomycin on the Proliferation of B16 Mouse Melanoma Cells In Vitro. Anticancer Res. 2021 Dec;41(12):6113-6121. doi: 10.21873/anticanres.15431. PMID: 34848466.
[6]. Yu C, Xing F, et,al. Anisomycin suppresses Jurkat T cell growth by the cell cycle-regulating proteins. Pharmacol Rep. 2013;65(2):435-44. doi: 10.1016/s1734-1140(13)71019-3. PMID: 23744428.
[7]. Ling L, Wen Y, et,al. Anisomycin inhibits the activity of human ovarian cancer stem cells via regulating antisense RNA NCBP2-AS2/MEK/ERK/STAT3 signaling. J Gene Med. 2024 Jan;26(1):e3571. doi: 10.1002/jgm.3571. Epub 2023 Jul 22. PMID: 37483091.
[8]. Yang W, Zhou C, et,al. Anisomycin inhibits angiogenesis, growth, and survival of triple-negative breast cancer through mitochondrial dysfunction, AMPK activation, and mTOR inhibition. Can J Physiol Pharmacol. 2022 Jul 1;100(7):612-620. doi: 10.1139/cjpp-2021-0577. Epub 2022 Jul 19. PMID: 35852219.
[9]. Hadwen J, Farooq F, et,al. Anisomycin Activates Utrophin Upregulation Through a p38 Signaling Pathway. Clin Transl Sci. 2018 Sep;11(5):506-512. doi: 10.1111/cts.12562. Epub 2018 Jun 7. PMID: 29877606; PMCID: PMC6132359.
| Cell experiment [1]: | |
Cell lines | B16 cells |
Preparation Method | Cells were seeded into a 96-well microplate (6×103 cells/well). After 24 h, the medium was changed to a fresh medium containing different concentrations of anisomycin, followed by further incubation for 24 h. |
Reaction Conditions | 0-100nM; 24h |
Applications | Anisomycin concentration higher than 10 nM led to a decrease in cell viability in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | Six-week-old SCID mice (MD-MBA-231 cells breast cancer model) |
Preparation Method | Treatments were initialized after 1 week of tumor inoculations. Anisomycin at 5 mg/kg or 100 µL PBS was administrated via intraperitoneal injection. |
Dosage form | 5 mg/kg; i.p; 3 weeks |
Applications | Anisomycin significantly suppresses growth of MDA-MB-231 tumor in vivo. |
References: | |
| Cas No. | 22862-76-6 | SDF | |
| المرادفات | Flagecidin, NSC 76712, Wuningmeisu C | ||
| Chemical Name | (2R,3S,4S)-4-hydroxy-2-(4-methoxybenzyl)pyrrolidin-3-yl acetate | ||
| Canonical SMILES | O[C@@H]1[C@H]([C@@H](CC(C=C2)=CC=C2OC)NC1)OC(C)=O | ||
| Formula | C14H19NO4 | M.Wt | 265.31 |
| الذوبان | ≥ 26.5mg/mL in DMSO | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7692 mL | 18.8459 mL | 37.6918 mL |
| 5 mM | 753.8 μL | 3.7692 mL | 7.5384 mL |
| 10 mM | 376.9 μL | 1.8846 mL | 3.7692 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
TIL-35 promoted microglial M2 polarization via suppressing JNK signaling. (A)Western blot was performed to determine protein levels of JNK and p-JNK.
HAPI cells were pre-treated with anisomycin (0.1μM; JNK signaling activator; Glpbio, Montclair, CA, USA).
Arch Biochem Biophys (2020): 108330. PMID: 32156533 IF: 4.01 -
Related Biological Data
TAK1 knockdown improves cell viability and alleviates the inflammation of H/R‑induced Caco‑2 cells. (B) Expression levels of TNF‑α, IL‑6 and IL‑1β in the five groups were determined using reverse transcription‑quan‑titative PCR.
Anisomycin, a type of JNK agonist, was purchased from GlpBio Technology and diluted to 4µM.
Exp Ther Med 23.2 (2022): 1-9. PMID: 35069839 IF: 2.443 -
Related Biological Data
Butorphanol decreases LPS‑induced apoptosis via p38/JNK/ATF2/p53 signaling. (A) Anisomycin increased the number of apoptotic cells.
Cells were pretreated with butorphanol (1, 2 and 4µM; GlpBio Technology) and/or anisomycin (1µM; GlpBio Technology Inc.).
Exp Ther Med 23.3 (2022): 1-9. PMID: 35222706 IF: 2.443 -
Related Biological Data
Knockdown of ANKRD49 downregulates MMP-2/MMP-9 expression of H1703 cells. (G, H) The levels of JNK, p-JNK, p38, p-p38, MMP-2 and MMP-9 in ANKRD49-sh H1703 cells treated with SP600125, SB203580, Anisomycin or DMSO were measured by Western blot. β-Tubulin served as an internal control. Data are expressed as means±standard deviation.
Anisomycin (20µM) (GlpBio, USA), an activator of JNK and p38 MAPK was used to pretreat ANKRD49-sh H1703 cells for 1h.
bioRxiv (2023): 2023-03. PMID: 37964204
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)