β-Elemene (Synonyms: (-)-β-Elemene,Levo-β-elemene) |
رقم الكتالوجGC19537
One-Click Copy Product Info
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β؛ -Elemene ((-) - β؛ -Elemene؛ Levo-β؛ -elemene) معزول عن نبات الكركم العطري الطبيعي مع نشاط مضاد للأورام. β ؛ -يمكن لإليمين إحداث موت الخلايا المبرمج.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 515-13-9
Sample solution is provided at 25 µL, 10mM.
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β-Elemene is a monoterpenoid compound extracted from the natural plant Curcuma aromatica, which has antitumor activity[1]. β-Elemene has antiproliferative effects on multiple cancer cells mainly by inducing apoptosis and blocking the cell cycle[2]. β-Elemene is a RhoA kinase inhibitor[3].
In vitro, β-Elemene (0.5, 5, 50μM) pretreated human umbilical vein endothelial cells (HUVECs) for 24h, and the inhibition rates of H2O2-induced cytotoxicity were 59.7%, 75.1% and 86.2%, respectively, reducing the production of reactive oxygen species (ROS) and preventing the activation of mitogen-activated protein kinase (MAPK) signaling pathway in cells[4]. β-Elemene (0-320μg/mL) treated A549 cells for 24-72h inhibited cell growth in a dose- and time-dependent manner, with an IC50 value of 27.5μg/mL[5]. Treatment of H460 cells with β-Elemene (0-50μg/mL) for 24h or 48h inhibited cell growth and induced cell cycle arrest and apoptosis[6].
In vivo, β-Elemene (20, 50mg/kg) was intraperitoneally injected into B16F10 cell xenograft mice, inhibiting B16F10 melanoma lung metastasis and the expression of vascular endothelial growth factor (VEGF)[7].
References:
[1] Zhang Y, Peng F, Yu C. Therapeutic potential of Curcuma oil and its terpenoids in gynecological cancers[J]. Biomedicine & Pharmacotherapy, 2023, 157: 114016.
[2] Zhai B, Zhang N, Han X, et al. Molecular targets of β-elemene, a herbal extract used in traditional Chinese medicine, and its potential role in cancer therapy: A review[J]. Biomedicine & Pharmacotherapy, 2019, 114: 108812.
[3] Wang J, Li H, Yao Y, et al. β-Elemene enhances GAP-43 expression and neurite outgrowth by inhibiting RhoA kinase activation in rats with spinal cord injury[J]. Neuroscience, 2018, 383: 12-21.
[4] Liu M, Mao L, Daoud A, et al. β-elemene inhibits monocyte–endothelial cells interactions via reactive oxygen species/MAPK/NF-κB signaling pathway in vitro[J]. European journal of pharmacology, 2015, 766: 37-45.
[5] Liu Y, Jiang Z, Zhou Y, et al. β-elemene regulates endoplasmic reticulum stress to induce the apoptosis of NSCLC cells through PERK/IRE1α/ATF6 pathway[J]. Biomedicine & Pharmacotherapy, 2017, 93: 490-497.
[6] Wang G, Li X, Huang F, et al. Antitumor effect of β-elemene in non-small-cell lung cancer cells is mediated via induction of cell cycle arrest and apoptotic cell death[J]. Cellular and Molecular Life Sciences CMLS, 2005, 62: 881-893.
[7] Chen W, Lu Y, Wu J, et al. Beta-elemene inhibits melanoma growth and metastasis via suppressing vascular endothelial growth factor-mediated angiogenesis[J]. Cancer chemotherapy and pharmacology, 2011, 67: 799-808.
| Cell experiment [1]: | |
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Cell lines |
Human umbilical vein endothelial cells (HUVECs) |
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Preparation Method |
HUVECs were seeded in 96-well plates at a density of 5×103 cells/well. After 24h growth, HUVECs were pre-treated with β-Elemene (0.5, 5, 50μM) for 24h at 37°C in 5% CO2 incubator. Then, HUVECs were treated with 0.5mM H2O2 for 2h. After that MTT (0.5%, 20μL) was added to the medium and cells were further incubated for 4h. The supernatant was removed and 100μL DMSO was added to dissolve the precipitate. Finally, the absorbance was measured spectrophotometrically at 570nm. |
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Reaction Conditions |
0.5, 5, 50μM; 24h |
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Applications |
The inhibition rates of β-Elemene (0.5, 5, and 50μM) on H2O2-induced cytotoxicity were 59.7%, 75.1%, and 86.2%, respectively, which can protect endothelial cells from H2O2-induced cytotoxicity. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6 mice |
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Preparation Method |
2.5×105 B16F10 cells were injected subcutaneously into the left footpad of each 6-week-old female C57BL/6 mice. The mice were evenly divided in 4 groups (6 mice/group). Except mice of the control and untreated groups with 0.1mL of saline, other two groups were injected intraperitoneally with β-Elemene (20 and 50mg/kg) once a day, starting from the day after injection of the B16F10 cells into the footpad. This continued until the mice were killed. The length and width of the tumor were measured every day. Approximately 3 weeks later, when tumors in control group reached a diameter of 1cm3, the tumor-bearing legs were amputated along with the left popliteal lymph node. The primary melanoma was carefully separated from the legs for size analysis and then fixed with formalin for immunohistochemistry assay. After an additional 21 days, the mice were killed and autopsied to count the metastatic colonies in the bilateral lungs, and the lung of mice was stored at -80°C refrigerator for melanin measurement. In addition, the serum was prepared before the mice were killed and stored at -80°C refrigerator. |
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Dosage form |
20、50mg/kg; i.p. |
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Applications |
β-Elemene inhibited primary melanoma growth and angiogenesis. β-Elemene inhibited B16F10 melanoma lung metastasis. β-Elemene inhibited VEGF in vivo. |
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References: |
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| Cas No. | 515-13-9 | SDF | |
| المرادفات | (-)-β-Elemene,Levo-β-elemene | ||
| Chemical Name | (1S,2S,4R)-1-ethenyl-1-methyl-2,4-bis(1-methylethenyl)-cyclohexane | ||
| Canonical SMILES | C=C[C@@]1(C)[C@H](C(C)=C)C[C@H](C(C)=C)CC1 | ||
| Formula | C15H24 | M.Wt | 204.4 |
| الذوبان | DMF: 30 mg/mL;DMSO: 30 mg/mL;Ethanol: 30 mg/mL | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8924 mL | 24.4618 mL | 48.9237 mL |
| 5 mM | 978.5 μL | 4.8924 mL | 9.7847 mL |
| 10 mM | 489.2 μL | 2.4462 mL | 4.8924 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)