Janelia Fluor 549, free acid (Synonyms: JF549 TFA) |
| رقم الكتالوجGC50479 |
Janelia Fluor 549، free acid (JF549 TFA) هو صبغة فلورية بأقصى امتصاص (&Lambda؛ ab (max)) يبلغ 549 نانومتر وأقصى انبعاث (&Lambda؛ em (max)) يبلغ 571 نانومتر.
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Cas No.: 2245946-45-4
Sample solution is provided at 25 µL, 10mM.
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Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668
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