Mirin |
| رقم الكتالوجGC13491 |
ميرين هو مثبط جزيء صغير لمركب MRN (Mre11 و Rad50 و Nbs1).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1198097-97-0
Sample solution is provided at 25 µL, 10mM.
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Mirin is an inhibitor of Mre11-Rad50-Nbs1 (MRN) complex (IC50=12μM), which can inhibit the activity of MRE11-related exonuclease, and can inhibit the activation of MRN-dependent ATM[1]. It is commonly used in the study of cancers related to DNA repair mechanisms[1].
Mirin (100µM, 24 hours) significantly enhanced the sensitivity of human papillomavirus (HPV) to N-methylpyrrole-imidazole hairpin polyamide (PA25)[2]. Mirin (50μM, 24h) significantly enhanced DNA double-strand breaks and promoted cell apoptosis in esophageal squamous cell carcinoma (ESCC) cells treated with NU7441 and ionizing radiation treatment[3].
Mirin (50mg/kg/day, 9 days, intratumoral injection) efficiently restrains tumor growth in Neuroblastoma mouse model by inducing DNA damage response (DDR) and apoptosis[4]. Mirin (50mg/kg, 72 hours, i.p.) significantly increased tubular damage and serum markers of acute kidney injury (AKI) (creatinine and neutrophil gelatinase-associated lipocalin) in cisplatin-induced nephrotoxic AKI mouse model[5].
References:
[1] Dupré A, Boyer-Chatenet L, Sattler RM, et al. A forward chemical genetic screen reveals an inhibitor of the Mre11–Rad50–Nbs1 complex. Nature chemical biology. 2008 Feb;4(2):119-25.
[2] Edwards TG, Vidmar TJ, Koeller K, et al. DNA damage repair genes controlling human papillomavirus (HPV) episome levels under conditions of stability and extreme instability. PloS one. 2013 Oct 2;8(10):e75406.
[3] Wang G, Guo S, Zhang W, et al. A comprehensive analysis of alterations in DNA damage repair pathways reveals a potential way to enhance the radio-sensitivity of esophageal squamous cell cancer. Frontiers in oncology. 2020 Oct 16;10:575711.
[4] Petroni M, Sardina F, Infante P, et al. MRE11 inhibition highlights a replication stress-dependent vulnerability of MYCN-driven tumors. Cell death & disease. 2018 Aug 30;9(9):895.
[5] Hama T, Nagesh PK, Chowdhury P, et al. DNA damage is overcome by TRIP13 overexpression during cisplatin nephrotoxicity. JCI insight. 2021 Nov 11;6(22).
| Cell experiment [1]: | |
Cell lines | W12E cells |
Preparation Method | W12E cells were pre-treated with 100µM Mirin or 0.1% DMSO for 24h. Media was removed and fresh media containing the indicated doses of N-methylpyrrole-imidazole hairpin polyamide (PA25) or 0.1% DMSO was added, and cells incubated an additional 24h. |
Reaction Conditions | 100µM, 24 hours |
Applications | Mirin significantly sensitized human papillomavirus (HPV) episomes to PA25. |
| Animal experiment [2]: | |
Animal models | Neuroblastoma mouse model |
Preparation Method | 1. Mirin encapsulation in biocompatible polymeric nanocarriers: |
Dosage form | 50mg/kg/d, 9 days, intratumoral injection |
Applications | Mirin efficiently restrains tumor growth in vivo by inducing DNA damage response (DDR) and apoptosis. |
| Cas No. | 1198097-97-0 | SDF | |
| Chemical Name | (Z)-5-(4-hydroxybenzylidene)-2-imino-2,5-dihydrothiazol-4-ol | ||
| Canonical SMILES | N=C1N=C(O)/C(S1)=C([H])/C2=CC=C(O)C=C2 | ||
| Formula | C10H8N2O2S | M.Wt | 220.25 |
| الذوبان | ≥ 9.3mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.5403 mL | 22.7015 mL | 45.403 mL |
| 5 mM | 0.9081 mL | 4.5403 mL | 9.0806 mL |
| 10 mM | 0.454 mL | 2.2701 mL | 4.5403 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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