ML-346 |
| رقم الكتالوجGC18170 |
ML-346 هو منشط لتعبير Hsp70 ونشاط HSF-1 ، مع EC50 من 4.6 ميكرومتر لـ Hsp70. يستعيد ML-346 طي البروتين في نماذج الأمراض التوافقية ، دون سمية خلوية كبيرة أو نقص في الخصوصية. يحث ML-346 على زيادات محددة في الجينات ومؤثرات البروتين لاستجابة الصدمة الحرارية (HSR) ، بما في ذلك المرافقون مثل Hsp70 و Hsp40 و Hsp27.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 100872-83-1
Sample solution is provided at 25 µL, 10mM.
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ML-346 is an activator of the heat shock response that induces the expression of the heat shock proteins HSP70, HSP40, and HSP27.[1] It induces the expression of the oxidative stress response genes HO1, GCLM, and BiP in mouse embryonic fibroblasts (MEFs) and pretreatment protects cells from severe heat shock-induced death and H2O2-induced apoptosis. It promotes folding of metastable proteins in models of conformational disease, including cellular models of Huntingtin aggregation and cystic fibrosis. It reduces aggregate formation in PC12 cells expressing human Huntingtin exon 1 containing a 74 glutamine expansion when used at a concentration of 10 uM. It also corrects trafficking of cystic fibrosis transmembrane conductance regulator proteins bearing the F508 deletion (F508-CFTR) mutation carried by the majority of cystic fibrosis patients, leading to increased cell surface expression.
References
[1]. Calamini, B., Silva, M.C., Madoux, F., et al. Small-molecule proteostasis regulators for protein conformational diseases. Nat. Chem. Biol. 8(2), 185-196 (2011).
| Cas No. | 100872-83-1 | SDF | |
| Chemical Name | 5-[3-(4-methoxyphenyl)-2-propen-1-ylidene]-2,4,6(1H,3H,5H)-pyrimidinetrione | ||
| Canonical SMILES | O=C1NC(/C(C(N1)=O)=C/C=C/C2=CC=C(OC)C=C2)=O | ||
| Formula | C14H12N2O4 | M.Wt | 272.3 |
| الذوبان | 0.5mg/mL in ethanol, 30mg/mL in DMSO, or in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL |
| 5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL |
| 10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)
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