Neferine (Synonyms: 4′′-O-Methylliensinine, (-)-Neferine) |
| رقم الكتالوجGN10103 |
Neferine (NEF) is a natural bisbenzylisoquinoline alkaloid separated from the traditional Chinese medicine Nelumbinis plumula. Neferine has a variety of pharmacological properties.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2292-16-2
Sample solution is provided at 25 µL, 10mM.
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Neferine (NEF) is a natural bisbenzylisoquinoline alkaloid separated from the traditional Chinese medicine Nelumbinis plumula. Neferine has a variety of pharmacological properties[1].
In vitro, Neferine (1, 3 and 10μM; 20min) inhibited the expression of cytokines and chemokines in TNF-α/IFN-γ-stimulated human keratinocyte (HaCaT) cells, and Neferine reduced the phosphorylation of MAPK and the NF-κB signaling pathway[2]. Neferine (12.5 or 25μM; 24h) enhanced the AMPK and ACC phosphorylation in OA-stimulated HepG2 cells [3].
In vivo, Neferine significantly alleviated DNCB-induced skin barrier damage, scratching responses, and epidermal hyperplasia in mice. And Neferine significantly decreased transepidermal water loss (TEWL), erythema, blood flow, and ear thickness and increased surface skin hydration[2]. Neferine (5mg/kg/day or 10mg/kg/day; 4 weeks; i.p.) treatment mitigated hepatic lipid deposition, inflammatory cell infiltration as well as hepatic fibrosis[3]. Neferine (20mg/kg; 30 days; i.p.) inhibits tumorigenesis and induces ferroptosis in nude mice[4].
References:
[1] Marthandam Asokan S, Mariappan R, Muthusamy S, Velmurugan BK. Pharmacological benefits of neferine - A comprehensive review. Life Sci. 2018 Apr 15;199:60-70.
[2] Yang CC, Hung YL, Ko WC, Tsai YJ, Chang JF, Liang CW, Chang DC, Hung CF. Effect of Neferine on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice. Int J Mol Sci. 2021 Jul 30;22(15):8237.
[3] Wang MY, Zhang SS, An MF, Xia YF, Fan MS, Sun ZR, Zhang LJ, Zhao YL, Sheng J, Wang XJ. Neferine ameliorates nonalcoholic steatohepatitis through regulating AMPK pathway. Phytomedicine. 2023 Jun;114:154798.
[4] Li S, Zhang Y, Zhang J, Yu B, Wang W, Jia B, Chang J, Liu J. Neferine Exerts Ferroptosis-Inducing Effect and Antitumor Effect on Thyroid Cancer through Nrf2/HO-1/NQO1 Inhibition. J Oncol. 2022 Jun 27;2022:7933775.
| Cell experiment [1]: | |
Cell lines | HepG2 cells |
Preparation Method | HepG2 cells were plated on glass coverslips in 12-well plates and treated with OA (0.5mM) for 24h in the absence or presence of Neferine (12.5 or 25µM). The cells were harvested and rinsed twice with phosphate-buffered saline (PBS). The cells were then fixed with 4% paraformaldehyde at room temperature for 20min. The cells were rinsed with PBS 2-3 times at room temperature for 5min and stained with Oil Red O working solution (60% Oil Red O dye and 40% distilled water) at room temperature for 20min. The cells were observed under a light microscope. |
Reaction Conditions | 12.5 or 25µM; 24h |
Applications | Neferine (12.5 or 25μM; 24h) enhanced the AMPK and ACC phosphorylation in OA-stimulated HepG2 cells. |
| Animal experiment [2]: | |
Animal models | 4-week-old BALB/c nude mice |
Preparation Method | Ten nude mice were subcutaneously inoculated with a total of 2 × 10⁶ IHH-4 cells and then randomly divided into two groups: a control group and a Neferine 20mg/kg group. Mice in the Neferine 20mg/kg group were intraperitoneally injected with 20mg/kg Neferine, while those in the control group were given the same amount of dimethyl sulfoxide (DMSO). The tumor volume and mouse body weight were monitored every five days. After 30 days of consecutive monitoring, the mice were euthanized via intraperitoneal injection of sodium pentobarbital (100mg/kg), and the tumor samples were removed, weighed, and stored for subsequent examination. The tumor volume was calculated using the following formula: volume = 1/2 × length × width. |
Dosage form | 20mg/kg; 30 days; i.p. |
Applications | Neferine (20mg/kg; 30 days; i.p.) inhibits tumorigenesis and induces ferroptosis in nude mice. |
References: | |
| Cas No. | 2292-16-2 | SDF | |
| المرادفات | 4′′-O-Methylliensinine, (-)-Neferine | ||
| Chemical Name | 4-[[(1R)-6,7-dimethoxy-2-methyl-3,4-dihydro-1H-isoquinolin-1-yl]methyl]-2-[[(1R)-6-methoxy-1-[(4-methoxyphenyl)methyl]-2-methyl-3,4-dihydro-1H-isoquinolin-7-yl]oxy]phenol | ||
| Canonical SMILES | CN1CCC2=CC(=C(C=C2C1CC3=CC=C(C=C3)OC)OC4=C(C=CC(=C4)CC5C6=CC(=C(C=C6CCN5C)OC)OC)O)OC | ||
| Formula | C38H44N2O6 | M.Wt | 624.77 |
| الذوبان | DMSO : 125 mg/mL (200.07 mM; Need ultrasonic) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6006 mL | 8.0029 mL | 16.0059 mL |
| 5 mM | 320.1 μL | 1.6006 mL | 3.2012 mL |
| 10 mM | 160.1 μL | 800.3 μL | 1.6006 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.50%
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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