1-Methyladenine |
Catalog No.GC33646
One-Click Copy Product Info
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La 1-metiladenina es un producto del daÑo por alquilaciÓn en el ADN que puede repararse mediante la reversiÓn del daÑo por desmetilaciÓn oxidativa.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5142-22-3
Sample solution is provided at 25 µL, 10mM.
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1-Methyladenine is a methylated adenine nucleotide and a product of alkylation damage in DNA[1-2]. 1-Methyladenine is used in research related to DNA damage and developmental biology[3-4].
In vitro, diploid yeast Saccharomyces cerevisiae BZ34 (adenine auxotroph) was treated with 1-Methyladenine (20mg/L) for 24–48 hours. 1-Methyladenine completely inhibited cell growth, but did not cause cell death or induce cytotoxic or genotoxic effects[5]. Starfish oocytes were treated with 1-Methyladenine (1µM) for 3–10 minutes. 1-Methyladenine induced germinal vesicle breakdown (GVBD). When starfish oocytes were co-treated with 1-Methyladenine and the calcium ionophore A23187 (20µM), the formation of the fertilization envelope was completely suppressed, and the oocyte’s ability to form a fertilization envelope in response to elevated calcium was blocked[6].
References:
[1] Mita M. 1-Methyladenine: a starfish oocyte maturation-inducing substance. Zygote. 2000;8 Suppl 1:S9-11.
[2] Yuan L, Mao LH, Li JY. CAG Repeat Expansions Increase N1-Methyladenine to Alter TDP-43 Phase Separation: Lights Up Therapeutic Intervention for Neurodegeneration. Aging Dis. 2024 Mar 14;16(1):1–4.
[3] Mita M, Yoshikuni M, Nagahama Y. 1-Methyladenine production from ATP by starfish ovarian follicle cells. Biochim Biophys Acta. 1999 Jun 28;1428(1):13-20.
[4] DUNN DB. The occurrence of 1-methyladenine in ribonucleic acid. Biochim Biophys Acta. 1961 Jan 1;46:198-200.
[5] Murthy, M. Sreedhara, V. V Deorukhakar. Failure of exogenous methylated adenines to induce cytotoxic and genotoxic effects in yeast auxotrophic to adenine. Journal of Biosciences. 1985. 9: 223-229.
[6] Aida S, Matsumoto T, Yamazaki Y, et al. Rac1 Temporarily Suppresses Fertilization Envelope Formation Immediately After 1-Methyladenine Stimulation. Cells. 2025 Mar 10;14(6):405.
| Cell experiment [1]: | |
Cell lines | Diploid yeast Saccharomyces cerevisiae BZ34 (adenine auxotroph) |
Preparation Method | Yeast cells from stationary phase culture were washed and resuspended in synthetic complete (SC) medium in which adenine was replaced by 1-Methyladenine (20mg/L). Cultures were incubated in a shaker water bath at 30°C. Growth was monitored at 24 and 48 hours. |
Reaction Conditions | 20mg/L; 24-48h |
Applications | 1-Methyladenine completely inhibited cell growth (without causing cell death) and did not induce cytotoxic or genotoxic effects, as measured by the frequency of gene conversion and reverse mutation. |
References: | |
| Cas No. | 5142-22-3 | SDF | |
| Canonical SMILES | NC1=C2N=CN=C2N=CN1C | ||
| Formula | C6H7N5 | M.Wt | 149.15 |
| Solubility | Water: 5.2 mg/mL (34.86 mM; ultrasonic and warming and heat to 80°C) | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL |
| 10 mM | 670.5 μL | 3.3523 mL | 6.7047 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)