10Panx (Synonyms: 10Panx) |
Catalog No.GC13863
One-Click Copy Product Info
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10Panx is a potent Panx1 channel inhibitor that targets the W74 to Y83 segment of the ECL1 of Panx1 protein.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 955091-53-9
Sample solution is provided at 25 µL, 10mM.
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10Panx is a potent Panx1 channel inhibitor that targets the W74 to Y83 segment of the ECL1 of Panx1 protein [1]. 10Panx inhibits the P2X7 receptor activation-induced cleavage of caspase-1 and the processing and release of IL-1β by suppressing Panx1[2]. 10Panx has been widely used to alleviate the characteristic inflammatory responses in mouse models of asthma[3].
In vitro, 10Panx (50μM) treatment for 20 hours significantly inhibited the formation of capillary-like structures in human dermal lymphatic endothelial cells (HDLECs)[4]. Treatment with 10Panx at a concentration of 100μM for 6 hours delays the maturation of bovine oocytes and reduces the level of reactive oxygen species within the cells[5]. The 72h treatment of HCT116 cells with 200μM 10Panx significantly inhibited cell proliferation[6].
In vivo, 10Panx treatment via intraventricular injection of 5μl (2μg/μl) 0.5 hours before middle cerebral artery occlusion (MCAO) can reduce the infarct volume in rats and alleviate neurological deficits[7]. A single intravenous injection of 2.58nmol/g dose of 10Panx reduced the induced dentin hypersensitivity of the first molar in rats[8].
References:
[1] Jiang M, Li X, Xie K. Pannexin channels in inflammation and tumorigenesis[J]. Frontiers in Cell and Developmental Biology, 2025, 13: 1647765.
[2] Pelegrin P, Surprenant A. Pannexin‐1 mediates large pore formation and interleukin‐1β release by the ATP‐gated P2X7 receptor[J]. The EMBO journal, 2006, 25(21): 5071-5082.
[3] Martinez L A, Rasky A J, Morris S B, et al. Inhibition of Pannexin1 hemichannels in the airways epithelium reduces the characteristic inflammatory response in an asthmatic mouse model[J]. World Allergy Organization Journal, 2020, 13(8).
[4] Boucher J, Simonneau C, Denet G, et al. Pannexin-1 in human lymphatic endothelial cells regulates lymphangiogenesis[J]. International Journal of Molecular Sciences, 2018, 19(6): 1558.
[5] Dye Z T, Rutledge L V, Penuela S, et al. Pannexin 1 inhibition delays maturation and improves development of Bos taurus oocytes[J]. Journal of Ovarian Research, 2020, 13(1): 98.
[6] Fierro-Arenas A, Landskron G, Camhi-Vainroj I, et al. Pannexin-1 expression in tumor cells correlates with colon cancer progression and survival[J]. Life Sciences, 2024, 351: 122851.
[7] Wei R, Bao W, He F, et al. Pannexin1 channel inhibitor (10panx) protects against transient focal cerebral ischemic injury by inhibiting RIP3 expression and inflammatory response in rats[J]. Neuroscience, 2020, 437: 23-33.
[8] Ohyama S, Ouchi T, Kimura M, et al. Piezo1-pannexin-1-P2X3 axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity[J]. Frontiers in physiology, 2022, 13: 891759.
| Cell experiment [1]: | |
Cell lines | HCT116 cells |
Preparation Method | HCT116 cells were grown in DMEM medium with 10% (v/v) fetal bovine serum (FBS), 100μg/ml streptomycin, and 100U/ml penicillin at 37°C in 5% CO2/atmosphere. HCT116 cells were seeded in triplicates at a density of 1×104 cells per well in the 96-well plates containing 100μl culture medium (1% FBS) and allowed to adhere in a 5% CO2 incubator at 37°C overnight. Cells were treated with 200μM 10Panx or PBS (vehicle) for 24, 48 and 72h. Cell viability was evaluated. |
Reaction Conditions | 200µM; 24, 48 and 72h |
Applications | 10Panx treatment significantly reduced the cell viability of HCT116 cells in a time-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male Sprague-Dawley rats |
Preparation Method | Male Sprague-Dawley rats (250-280g) were housed singly in a standard environment with food and water ad libitum. A total of 80 rats were randomly assigned to 3 groups and treated with or without 10Panx: (1) in the sham group (n = 14), rats received anesthesia and vehicle injection; (2) in the surgery plus vehicle group, rats underwent MCAO under isoflurane anesthesia, and vehicle was administered intracerebroventricularly 30min before ischemia onset (n = 22) ; and (3) in the surgery plus 10Panx group, rats were administered intracerebroventricular injections of 10Panx (2μg/μl; 5μl) at 0.5h before MCAO under isoflurane anesthesia. Rats were sacrificed 48 hours after MCAO for experiments and the brain tissues were collected for analysis. |
Dosage form | 2μg/μl (5μl) for once; i.c.v. |
Applications | 10Panx treatment significantly reduced the infarct volume and alleviate neurological deficits in MCAO model of rats. |
References: | |
| Cas No. | 955091-53-9 | SDF | |
| Sinónimos | 10Panx | ||
| Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC2=CC=CC=C2)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC3=CNC4=CC=CC=C43)N | ||
| Formula | C58H79N15O16 | M.Wt | 1242.37 |
| Solubility | ≥ 124.2mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 804.9 μL | 4.0246 mL | 8.0491 mL |
| 5 mM | 161 μL | 804.9 μL | 1.6098 mL |
| 10 mM | 80.5 μL | 402.5 μL | 804.9 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)