Acevaltrate |
Catalog No.GC30927
One-Click Copy Product Info
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Acevaltrate inhibe la actividad de Na+/K+-ATPasa en los hemisferios renal y cerebral de rata con IC50 de 22,8 μM y 42,3 μM, respectivamente.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 25161-41-5
Sample solution is provided at 25 µL, 10mM.
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Acevaltrate is an active component extracted from the roots of Valeriana glechomifolia,which inhibits Na+/K+-ATPase in the rat kidney and brain hemispheres with IC50s of 22.8μM and 42.3μM[1, 2]. Acevaltrate has antitumor effect on various cancer types, and can induce cell apoptosis and inhibit proliferation through the HIF-1α, Otub1/c-Maf, and USP10/CCND1 axes[3, 4]. Acevaltrate can inhibit A549, PC-3M, HCT-8, and Bel7402 cell lines with IC50 values of 2.9µM, 1.4µM, 1.0µM, and 1.7µM, respectively[5].
In vitro, Acevaltrate (0, 2 and 4μM; 24h) downregulated CCND1 protein expression in glioblastoma cells in a concentration-dependent manner[4]. Acevaltrate (0, 1, 2 and 5μM; 1h) significantly downregulated PCBP1 and PCBP2 proteins in a dose-dependent manner in RKO cells[6].
In vivo, Acevaltrate (0, 10, 25, and 50mg/kg; 22d; p.o.) suppressed tumor growth in nude mice inoculated with RKO cells[6].
References:[1] Mi C, Zhang Q L, Sun M J, et al. Acevaltrate promotes apoptosis and inhibits proliferation by suppressing HIF-1α accumulation in cancer cells [J]. International immunopharmacology, 2024, 133: 112066.
[2] Bettero G M, Salles L, Rosário Figueira R M, et al. In vitro effect of valepotriates isolated from Valeriana glechomifolia on rat P-type ATPases [J]. Planta medica, 2011, 77(15): 1702-1706.
[3] Sun T, Xu Y, Xu Z, et al. Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis [J]. Cell communication and signaling, 2021, 19(1): 24.
[4] Sun T, Xu Y J, Jiang S Y, et al. Suppression of the USP10/CCND1 axis induces glioblastoma cell apoptosis [J]. Acta pharmacologica Sinica, 2021, 42(8): 1338-1346.
[5] Lin S, Shen Y-H, Li H-L, et al. Acylated iridoids with cytotoxicity from Valeriana jatamansi [J]. Journal of natural products, 2009, 72(4): 650-655.
[6] Yu D, Hu H, Zhang Q, et al. Acevaltrate as a novel ferroptosis inducer with dual targets of PCBP1/2 and GPX4 in colorectal cancer [J]. Signal transduction and targeted therapy, 2025, 10(1): 211.
| Cell experiment [1]: | |
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Cell lines |
Glioblastoma cell lines (U251) |
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Preparation Method |
Cells were seeded in 96-well plates and treated with Acevaltrate for 24h. Then the cells were subjected to Annexin V/PI staining and flow cytometric analyses, and the cell lysates were subjected to immunoblot assays against PARP, Caspase-3 and pro-survival proteins. |
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Reaction Conditions |
0, 2 and 4μM; 24h |
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Applications |
Acevaltrate induced 40% cell death at 4μM, caused PARP and Caspase-3 cleavage, and downregulated the expression of Bcl-2 and Mcl-1 in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Male nude mice |
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Preparation Method |
Mice were subcutaneously inoculated with RKO cells (5×106 cells). After subcutaneous inoculation of tumor cells, mice were randomly grouped according to tumor volume and body weight when the tumor volume reached 50mm3. Acevaltrate was orally administered at doses of 0, 10, 25, and 50mg/kg once daily for 22 days (corn oil as a control). Tumor size intensity was measured every 2-3days. |
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Dosage form |
0, 10, 25 and 50mg/kg; 22d; p.o. |
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Applications |
Acevaltrate treatment at 10, 25 and 50mg/kg significantly reduced tumor size, volume, and weight compared to the control group. |
| [1] Sun T, Xu Y J, Jiang S Y, et al. Suppression of the USP10/CCND1 axis induces glioblastoma cell apoptosis [J]. Acta pharmacologica Sinica, 2021, 42(8): 1338-1346. [2] Yu D, Hu H, Zhang Q, et al. Acevaltrate as a novel ferroptosis inducer with dual targets of PCBP1/2 and GPX4 in colorectal cancer [J]. Signal transduction and targeted therapy, 2025, 10(1): 211. | |
| Cas No. | 25161-41-5 | SDF | |
| Canonical SMILES | O=C(CC(C)(C)OC(C)=O)O[C@@H]1[C@]2(CO2)[C@]([C@@H]3OC(CC(C)C)=O)([H])C(C(COC(C)=O)=CO3)=C1 | ||
| Formula | C24H32O10 | M.Wt | 480.5 |
| Solubility | DMSO : ≥ 125 mg/mL (260.15 mM) | Storage | Store at -20°C,protect from light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0812 mL | 10.4058 mL | 20.8117 mL |
| 5 mM | 416.2 μL | 2.0812 mL | 4.1623 mL |
| 10 mM | 208.1 μL | 1.0406 mL | 2.0812 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)