AK59 |
Catalog No.GC74005
One-Click Copy Product Info
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AK59 actúa como degradador de la proteína STING y se apoya en la ligasa E3 HERC4 con HECT-domain para cumplir su función.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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AK59 actúa como degradador de la proteína STING y se apoya en la ligasa E3 HERC4 con HECT-domain para cumplir su función[1]. Este compuesto acelera la degradación de STING vía el sistema ubiquitina-proteasoma, con lo que logra bloquear la vía de señalización cGAS/STING[2]. En ensayos bioquímicos suele emplearse AK59, ideal para filtrar inhibidores de STING y agentes degradantes dirigidos exclusivamente a variantes mutadas de STING[3].
| Experimento celular [1]: | |
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Líneas celulares |
Células THP1 |
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Método de preparación |
Las células THP1 se cultivaron en un medio RPMI 1640 que contenía un 10% de suero bovino fetal (FBS), 2mM de l-glutamine, 25mM de Hepes, 1mM de sodium pyruvate y 50U/ml de penicillin y 100mg/ml de streptomycin a 37℃ en presencia de 5% de CO2. 2×106 por pozo en una placa de seis pocillos se sembraron y trataron con diferentes concentraciones de AK59 (1, 2, 5, 7.5, 10µM) durante 16h. Posteriormente, se realizó un análisis de Western blot. |
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Condiciones de reacción |
1, 2, 5, 7.5, 10µM; 16h |
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Aplicaciones |
El tratamiento con AK59 redujo la expresión de la proteína STING en las células THP1 de manera dependiente de la dosis.. |
| Cas No. | SDF | ||
| Formula | C21H14F3N3O2S | M.Wt | 429.41 |
| Solubility | DMSO : 116.67 mg/mL (271.70 mM; Need ultrasonic) | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3288 mL | 11.6439 mL | 23.2878 mL |
| 5 mM | 465.8 μL | 2.3288 mL | 4.6576 mL |
| 10 mM | 232.9 μL | 1.1644 mL | 2.3288 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)